EFHD2 Antibody

Images

 
Western Blot: EFHD2 Antibody [NBP1-77135] - Analysis of EFHD2 in mouse brain tissue lysate with EFHD2 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: EFHD2 Antibody [NBP1-77135] - Immunofluorescence of EFHD2 in human brain tissue with EFHD2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: EFHD2 Antibody [NBP1-77135] - Human brain tissue with EFHD2 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EFHD2 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human EFHD2. The immunogen is located within the first 50 amino acids of EFHD2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EFHD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
EFHD2 Peptide (NBP1-77135PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-77135 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EFHD2 Antibody

  • EF hand domain containing 2
  • EF hand domain family, member D2
  • EF-hand domain family, member D2
  • EF-hand domain-containing protein D2
  • MGC4342
  • swiprosin 1
  • swiprosin-1
  • SWS1

Background

EFHD2, also known as Swiprosin-1 or SWS1, is an EF-hand and coiled-coil-containing adaptor protein that plays a role in lymphocyte physiology. EFHD2 exhibits the highest expression in CD8+ T cells and immature B cells. It provides a membrane scaffold that is required for the Syk-, SLP-65-, and PLCgamma2-dependent B-cell receptor (BCR)-induced calcium flux. EFHD2 may also regulate BCR-induced immature and primary B-cell apoptosis. It controls spontaneous apoptosis through the regulation of BCL2L1 abundance. Also, EFHD2 plays a role as negative regulator of the canonical NF-kappaB-activating branch.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-77135
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

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Review for EFHD2 Antibody (NBP1-77135) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: mice .
We have 1 review tested in 1 application: WB.

Reviews using NBP1-77135:
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WB
(1)
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mice
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB mice 04/27/2017
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Summary

ApplicationWestern Blot
Sample TestedHippocampal lysates from p20 mice
Speciesmice

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EFHD2 Antibody (NBP1-77135) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on EFHD2

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Recent Reviews

5
5
1
4
0
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0
2
0
1
0

 
Anonymous
04/27/2017
Application: WB
Species: mice

Bioinformatics

Gene Symbol EFHD2
Entrez
Uniprot