EFEMP2 Overexpression Lysate (Native)

Images

 
Western Blot: EFEMP2 Overexpression Lysate (Adult Normal) [NBL1-10136] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EFEMP2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EFEMP2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203726 and based on accession number NM_016938. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2), mRNA.
Gene
EFEMP2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
49.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for EFEMP2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EFEMP2 Overexpression Lysate (Native)

  • EGF containing fibulin-like extracellular matrix protein 2
  • EGF-containing fibulin-like extracellular matrix protein 2
  • FBLN4fibulin-4
  • FIBL-4
  • fibulin 4
  • Fibulin-4
  • MBP1
  • Protein UPH1
  • UPH1mutant p53 binding protein 1

Background

A large number of extracellular matrix proteins have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein encoded by this gene contains four EGF2 domains and six calcium-binding EGF2 domains. This gene is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol EFEMP2