EEF2K Antibody - BSA Free

Western Blot: EEF2K Antibody [NB100-87019] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-87019 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eEF2 Kinase was successfully immunoprecipitated by NB100-87018, which recognizes an upstream epitope.

Immunohistochemistry-Paraffin: EEF2K Antibody [NB100-87019] - Section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eEF2 Kinase used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

EEF2K Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit EEF2K Antibody - BSA Free (NB100-87019) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 675 and 725 of human calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase (EEF2K ) using the numbering given in entry NP_037434.1 (GeneID 29904).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EEF2K
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunoprecipitation Not recommended.
• Control:

  EEF2K Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EEF2K Antibody - BSA Free

• Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
• calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
• EC 2.7.11
• EC 2.7.11.20
• eEF-2 kinase
• eEF-2Kcalmodulin-dependent protein kinase III
• elongation factor-2 kinase
• eukaroytic elongation factor 2 kinase
• eukaryotic elongation factor 2 kinase
• eukaryotic elongation factor-2 kinase
• HSU93850
• MGC45041

Background

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EEF2K
• Entrez:
  • 29904(Human)
• Uniprot:
  • NP_037434.1(Human)