EEF1A2 Antibody - BSA Free

Western Blot: EEF1A2 Antibody [NBP2-97554] - Anti-EEF1A2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: U-251 MG Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa

Immunocytochemistry/Immunofluorescence: EEF1A2 Antibody [NBP2-97554] - Immunofluorescence staining of EEF1A2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human EEF1A2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-97554] - Immunochemical staining of human EEF1A2 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: EEF1A2 Antibody [NBP2-97554] - EEF1A2 was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate 4 ul anti-EEF1A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-EEF1A2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EEF1A2 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human EEF1A2 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EEF1A2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for EEF1A2 Antibody - BSA Free

• eEF1A-2
• EEF1ALFLJ41696
• EF1A
• EF-1-alpha-2
• elongation factor 1-alpha 2
• elongation factor-1 alpha
• Eukaryotic elongation factor 1 A-2
• eukaryotic translation elongation factor 1 alpha 2
• HS1
• statin S1
• statin
• statin-like
• statin-S1
• STN
• STNL

Background

EEF1A2 encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EEF1A2