EEF1A2 Antibody - BSA Free

Western Blot: EEF1A2 Antibody [NBP1-32555] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: EEF1A2 Antibody [NBP1-32555] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:5000.

Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP1-32555] - EEF1A2 antibody [C1C3] detects EEF1A2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.EEF1A2 stained by EEF1A2 antibody [C1C3] (NBP1-32555) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP1-32555] - EEF1A2 antibody [C1C3] detects EEF1A2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.EEF1A2 stained by EEF1A2 antibody [C1C3] (NBP1-32555) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: EEF1A2 Antibody [NBP1-32555] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody [C1C3] (NBP1-32555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Rb, Xp, Ze
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EEF1A2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit EEF1A2 Antibody - BSA Free (NBP1-32555) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human EEF1A2. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Zebrafish (95%), Bovine (100%), Rabbit (100%), Chicken (99%), Xenopus (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EEF1A2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for EEF1A2 Antibody - BSA Free

• eEF1A-2
• EEF1ALFLJ41696
• EF1A
• EF-1-alpha-2
• elongation factor 1-alpha 2
• elongation factor-1 alpha
• Eukaryotic elongation factor 1 A-2
• eukaryotic translation elongation factor 1 alpha 2
• HS1
• statin S1
• statin
• statin-like
• statin-S1
• STN
• STNL

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EEF1A2