EDNRA/Endothelin R Type A Antibody (485711)

EDNRA was detected in immersion fixed paraffin-embedded sections of human kidney using Human EDNRA Monoclonal Antibody (Catalog # MAB6538) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 485711
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

EDNRA/Endothelin R Type A Antibody (485711) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human EDRNA
  Accession # NP_001948
• Specificity: Detects human EDRNA in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EDNRA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EDNRA/Endothelin R Type A Antibody (485711)

• EDNRA
• endothelin receptor type A
• endothelin-1 receptor
• endothelin-1-specific receptor
• ET1-specific type
• ETAR
• ETRA
• G protein-coupled receptor
• hET-AR

Background

EDNRA (endothelin A receptor) is a 427 amino acid (aa) protein of the rhodopsin-like 7-transmembrane receptor family. It can be variably modified by N-linked glycoproteins, phosphorylation and palmitoylation.  It binds endothelins ET-1 and ET-2 preferentially, while EDNRB binds all three forms equally. Developmentally, EDNRA is expressed by cranial neural crest cells and induces facial morphogenesis. It is expressed by adult blood vessel smooth muscle cells, and is primarily responsible for the vasoconstrictor effects of ET-1. Human EDNRA shares 86% aa identity with mouse and rat EDNRA within the combined extracellular portions of the molecule.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EDNRA/Endothelin R Type A Antibody (MAB6538)(1)

Publication using MAB6538

• EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development Cell Stem Cell, 2020-09-17;0(0):. 2020-09-17 [PMID: 32946788] (IHC, Human)

Bioinformatics

• Gene Symbol: EDNRA
• Uniprot:
  • NP_001948()