EDEM1 Antibody

Immunocytochemistry/Immunofluorescence: EDEM1 Antibody [NBP1-84905] - Staining of human cell line RT4 shows localization to aggresome. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: EDEM1 Antibody [NBP1-84905] - Staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.

Product Discontinued

EDEM1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EDEM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for EDEM1 Antibody

• EDEMKIAA0212ER degradation-enhancing alpha-mannosidase-like 1
• ER degradation enhancer, mannosidase alpha-like 1
• FLJ51559
• FLJ51560

Background

Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-dependent manner. It lacks mannosidase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EDEM1