EDC3 Antibody - BSA Free

Immunoprecipitation:EDC3 AntibodyNBP3-29025] -Detection of human EDC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-EDC3 antibody NBP3-29025 (lot NBP3-29025-1) used for IP at 6 µg/mg lysate. EDC3 was also immunoprecipitated by rabbit anti-EDC3 antibody. For blotting immunoprecipitated EDC3, NBP3-29025 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EDC3 Antibody - BSA Free Summary

• Immunogen: Between 25 and 75
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EDC3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for EDC3 Antibody - BSA Free

• enhancer of mRNA decapping 3 homolog (S. cerevisiae)
• enhancer of mRNA-decapping protein 3
• FLJ21128
• FLJ31777
• hYjeF_N2
• hYjeF_N2-15q23
• LSM16 homolog (EDC3, S. cerevisiae)
• LSM16 homolog
• LSM16
• YJDC
• yjeF domain containing
• YjeF domain-containing protein 1
• YjeF N-terminal domain-containing protein 2
• yjeF_N2
• YJEFN2yjeF domain containing (E.coli)

Background

EDC3, also known as Enhancer of mRNA-decapping protein 3, is a 508 amino acid that is 56 kDa, located in the cytoplasm and expressed in theca and granulosa cells in ovary, Sertoli cells in testis (at protein level), brain and mammary glands; may play a role in mRNA decapping in the process of mRNA degradation, and also in spermiogenesis and oogenesis. Disease research is currently being studied with relation to central nervous system origin vertigo, patellofemoral pain syndrome and hordeolum. Interactions with the EDC3 protein have been shown to involve YWHAG, YWHAB, ZFP36, SFN, DDX6, YWHAZ, DCP2, UPF1, DCP1B and KBTBD7 in n exonucleolytic nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay, gene expression, and mRNA metabolic process pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EDC3