ECH1 Antibody

Images

 
Western Blot: ECH1 Antibody [NBP1-31662] - Sample (30 ug of whole cell lysate) A: 293T B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-31662] - Sample: Paraffin-embedded mouse intestine. ECH1 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-31662] - Paraffin-embedded OV90 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ECH1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ECH1. The exact sequence is proprietary.
Localization
Mitochondrion, Peroxisome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ECH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (82%), Pig (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ECH1 Antibody

  • delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
  • delta3,5-delta2,4-dienoyl-CoA isomerase
  • dienoyl-CoA isomerase
  • EC 5.3.3.-
  • enoyl CoA hydratase 1, peroxisomal
  • enoyl Coenzyme A hydratase 1, peroxisomal
  • HPXEL
  • peroxisomal enoyl-CoA hydratase 1

Background

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ECH1

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