EBF-3 Antibody

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse EBF-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EBF-3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

EBF-3 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse EBF-3
  Ile399-Ser504
  Accession # O08791-2
• Specificity: Detects endogenous human and mouse EBF-3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EBF3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBF-3 Antibody

• COE3
• COE3OE-2
• DKFZp667B0210
• early B-cell factor 3O/E-2
• EBF3
• EBF-3
• OE-2
• Olf-1/EBF-like 2
• transcription factor COE3

Background

EBF-3 (Early B cell Factor 3; also OE2 and COE3) is a 65 kDa (predicted) member of the COE family of transcription factors. It has limited expression, being identified principally in cerebellar Purkinje cells, olfactory neurons, and differentiating mantle layer neurons. Mouse EBF-3 is 596 amino acids (aa) in length it contains one DNA-binding region with an embedded zinc-finger motif (aa 51‑235), a dimerization segment between aa 371‑431, and a Pro/Ser-rich transactivation domain (aa 464‑555). EBF-3 either homodimerizes, or heterodimerizes with EBF-2 or -1. There are at least two potential splice variants, one that shows a nine aa deletion between aa 252 - 260, and a second that shows the same deletion coupled to a one aa substitution for aa 521‑557. Over aa 399‑504, mouse EBF-3 shows more than 99% aa identity to rat and human EBF-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBF-3 Antibody (AF5166)(2)

Publications using AF5166

• Mao Kuriki, Fuminori Sato, Hiroyuki N. Arai, Maina Sogabe, Mari Kaneko, Hiroshi Kiyonari et al. Transient and lineage-restricted requirement of Ebf3 for sternum ossification Development 2020-05-01 [PMID: 32398354]
• Mao Kuriki, Fuminori Sato, Hiroyuki N. Arai, Maina Sogabe, Mari Kaneko, Hiroshi Kiyonari et al. Transient and lineage-restricted requirement of Ebf3 for sternum ossification Development 2020-05-01 [PMID: 32398354] (Immunohistochemistry, Zebrafish)

Bioinformatics

• Gene Symbol: EBF3
• Uniprot:
  • O08791-2()