EBF-3 Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

EBF-3 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant mouse EBF-3
  Ile399-Ser504
  Accession # O08791-2
• Specificity: Detects endogenous human and mouse EBF-3 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBF-3 Antibody [Alexa Fluor® 750]

• COE3
• COE3OE-2
• DKFZp667B0210
• early B-cell factor 3O/E-2
• EBF3
• EBF-3
• OE-2
• Olf-1/EBF-like 2
• transcription factor COE3

Background

EBF-3 (Early B cell Factor 3; also OE2 and COE3) is a 65 kDa (predicted) member of the COE family of transcription factors. It has limited expression, being identified principally in cerebellar Purkinje cells, olfactory neurons, and differentiating mantle layer neurons. Mouse EBF-3 is 596 amino acids (aa) in length it contains one DNA-binding region with an embedded zinc-finger motif (aa 51‑235), a dimerization segment between aa 371‑431, and a Pro/Ser-rich transactivation domain (aa 464‑555). EBF-3 either homodimerizes, or heterodimerizes with EBF-2 or -1. There are at least two potential splice variants, one that shows a nine aa deletion between aa 252 - 260, and a second that shows the same deletion coupled to a one aa substitution for aa 521‑557. Over aa 399‑504, mouse EBF-3 shows more than 99% aa identity to rat and human EBF-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.