EBF-2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse EBF-2 Antigen ...read more
EBF-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) at 10 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

EBF-2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse EBF-2
Arg407-Ser519
Accession # O08792
Specificity
Detects mouse EBF-2 in direct ELISAs. Detects mouse and human EBF-2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
EBF2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF7006 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBF-2 Antibody [Unconjugated]

  • COE2
  • EBF2
  • EBF-2
  • Mmot1
  • O/E-3
  • OE-3

Background

EBF-2 (Early B cell Factor 2; also Mmot1, OLF3 and COE2) is a 62 kDa (predicted) member of the COE family of transcription factors. It is expressed in immature osteoblasts and Purkinje cells, and in the embryo is associated with the migration of postmitotic neuroblasts. In immature osteoblasts, EBF-2 appears to upregulate OPG, suppressing osteoclast formation. And in the developing retina, EBF-2 is found in ganglion, glycinergic Amacrine and horizontal cells, possibly promoting their development over that of photoreceptor cells. Mouse EBF-2 is 575 amino acids (aa) in length. It contains one DNA-binding region with an embedded C5-type zinc‑finger motif (aa 62-238), a dimerization ITP/TIG domain (aa 253-336), and a Pro/Ser-rich transactivation domain (aa 453-534). Although considered an HLH type transcription factor, it does not contain the typical "b", or basic amino acid sequence associated with bHLH factors. EBF-2 both homodimerizes, and heterodimerizes with EBF-1 and -3. There is an alternative start site at Met23. Over aa 407-519, mouse EBF-2 is identical in aa sequence to rat EBF-2 and shares 99% aa identity with human EBF-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EBF-2 Antibody (AF7006)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 6 applications: ChIP, Chromatin Immunoprecipitation (ChIP), ICC, Immunohistochemistry, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(1)
Chromatin Immunoprecipitation (ChIP)
(1)
ICC
(2)
Immunohistochemistry
(1)
Immunoprecipitation
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(5)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF7006 Applications Species
ES Liau, S Jin, YC Chen, WS Liu, M Calon, S Nedelec, Q Nie, JA Chen Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons Oncogene, 2023-01-03;14(1):46. 1/3/2023 [PMID: 36596814]
Cheuk Wang Fung, Shaopu Zhou, Han Zhu, Xiuqing Wei, Zhenguo Wu, Angela Ruohao Wu Cell fate determining molecular switches and signaling pathways in Pax7-expressing somitic mesoderm Cell Discovery 6/28/2022 [PMID: 35764624]
Margo P. Emont, Christopher Jacobs, Adam L. Essene, Deepti Pant, Danielle Tenen, Georgia Colleluori et al. A single-cell atlas of human and mouse white adipose tissue Nature 3/31/2022 [PMID: 35296864]
KM Choi, JH Kim, X Kong, M Isik, J Zhang, HW Lim, JC Yoon Defective brown adipose tissue thermogenesis and impaired glucose metabolism in mice lacking Letmd1 Cell Reports, 2021-12-14;37(11):110104. 2021-12-14 [PMID: 34910916] (Western Blot, Mouse, Transgenic Mouse) Western Blot Mouse, Transgenic Mouse
S Su, AR Guntur, DC Nguyen, SS Fakory, CC Doucette, C Leech, H Lotana, M Kelley, J Kohli, J Martino, S Sims-Lucas, L Liaw, C Vary, CJ Rosen, AC Brown A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome Cell Rep, 2018-12-11;25(11):3215-3228.e9. 2018-12-11 [PMID: 30540952] (ICC, Human) ICC Human
S Elattar, M Dimri, A Satyanaray The tumor secretory factor ZAG promotes white adipose tissue browning and energy wasting FASEB J., 2018-03-23;0(0):fj201701465RR. 2018-03-23 [PMID: 29570397] (Western Blot, Mouse) Western Blot Mouse
Yuta Hiraike, Hironori Waki, Jing Yu, Masahiro Nakamura, Kana Miyake, Gaku Nagano et al. NFIA co-localizes with PPAR gamma and transcriptionally controls the brown fat gene program Nature Cell Biology 9/1/2017 [PMID: 28812581]
Yuta Hiraike, Hironori Waki, Jing Yu, Masahiro Nakamura, Kana Miyake, Gaku Nagano et al. NFIA co-localizes with PPAR gamma and transcriptionally controls the brown fat gene program Nature Cell Biology 2017-09-01 [PMID: 28812581] (Chromatin Immunoprecipitation (ChIP), Mouse) Chromatin Immunoprecipitation (ChIP) Mouse
Suzanne N. Shapira, Hee-Woong Lim, Sona Rajakumari, Alexander P. Sakers, Jeff Ishibashi, Matthew J. Harms et al. EBF2 transcriptionally regulates brown adipogenesis via the histone reader DPF3 and the BAF chromatin remodeling complex Genes & Development 4/1/2017 [PMID: 28428261]
Binbin Lai, Ji-Eun Lee, Younghoon Jang, Lifeng Wang, Weiqun Peng, Kai Ge MLL3/MLL4 are required for CBP/p300 binding on enhancers and super-enhancer formation in brown adipogenesis Nucleic Acids Research 6/20/2017 [PMID: 28398509]
Show All 15 Publications.

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Bioinformatics

Gene Symbol EBF2
Uniprot