EBF-3 Antibody (8D6)

Western Blot: EBF-3 Antibody (8D6) [H00253738-M05] - Analysis of EBF3 expression in IMR-32 (Cat # L008V1).

Immunohistochemistry-Paraffin: EBF-3 Antibody (8D6) [H00253738-M05] - Analysis of monoclonal antibody to EBF3 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 0.3 ug/ml

Western Blot: EBF-3 Antibody (8D6) [H00253738-M05] - Analysis of EBF3 expression in NIH/3T3 (Cat # L018V1).

ELISA: EBF-3 Antibody (8D6) [H00253738-M05] - Detection limit for recombinant GST tagged EBF3 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 8D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EBF-3 Antibody (8D6) Summary

• Immunogen: EBF3 (NP_001005463, 381 a.a. ~ 480 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LYGMPHNNQEIILKRAADIAEALYSVPRNHNQIPTLGNNPAHTGMMGVNSFSSQLAVNVSETSQANDQVGYSRNTSSVSPRGYVPSSTPQQSNYNTVSTS
• Specificity: EBF3 (8D6)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EBF3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for ELISA and immunohistochemistry (paraffin).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29180763).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EBF-3 Antibody (8D6)

• COE3
• COE3OE-2
• DKFZp667B0210
• early B-cell factor 3O/E-2
• EBF3
• EBF-3
• OE-2
• Olf-1/EBF-like 2
• transcription factor COE3

Background

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (Bysimilarity)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBF-3 Antibody (H00253738-M05)(5)

Publications using H00253738-M05

• Jing L, Daniela O, Meriem S et al. A high-risk retinoblastoma subtype with stemness features, dedifferentiated cone states and neuronal/ganglion cell gene expression. Nat Commun. 2021-09-22 [PMID: 34552068]
• Tiklova K, Bjorklund AK, Lahti L et al. Single-cell RNA sequencing reveals midbrain dopamine neuron diversity emerging during mouse brain development. Nat Commun. 2019-02-04 [PMID: 30718509]
• Boufaied N, Nash C, Rochette A et al. Identification of genes expressed in a mesenchymal subset regulating prostate organogenesis using tissue and single cell transcriptomics Sci Rep. 2017-11-27 [PMID: 29180763] (IF/IHC, Rat)
• Moruzzo D, Nobbio L, Sterlini B et al. The Transcription Factors EBF1 and EBF2 Are Positive Regulators of Myelination in Schwann Cells. Mol Neurobiol 2016-11-26 [PMID: 27889898]
• Nasrallah MP, Cho G, Simonet JC et al. Differential effects of a polyalanine tract expansion in Arx on neural development and gene expression. Hum Mol Genet. 2012-03-01 [PMID: 22108177] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: EBF3
• Entrez:
  • 253738(Human)
• OMIM:
  • 607407(Human)
• Uniprot:
  • NP_001005463(Human)