EB3 [p Ser162] Antibody

Western Blot: EB3 [p Ser162] Antibody [NBP3-23299] - Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (NBP3-23294) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

EB3 [p Ser162] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-EB3 (Ser-162) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 162 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3, and is not conserved in EB1 and EB2. [UniProt# Q9UPY8]
• Modification: p Ser162
• Specificity: The antibody detects a 32 kDa protein corresponding to the apparent molecular mass of phosphorylated EB3 on SDS-PAGE immunoblots of mouse brain. This reactivity is significantly reduced after lambda phosphatase treatment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPRE3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for EB3 [p Ser162] Antibody

• EB1 protein family member 3
• EB3EBF3-S
• End-binding protein 3
• microtubule-associated protein RP/EB family member 3
• microtubule-associated protein, RP/EB family, member 3
• RP3EBF3APC binding protein

Background

EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli). It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPRE3