EAAT3 Antibody

Immunocytochemistry/Immunofluorescence: EAAT3 Antibody [NBP3-17050] - Analysis in human small intestine and testis tissues using Anti-SLC1A1 antibody. Corresponding SLC1A1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: EAAT3 Antibody [NBP3-17050] - Staining of human small intestine shows high expression.

Immunohistochemistry-Paraffin: EAAT3 Antibody [NBP3-17050] - Staining of human testis shows low expression as expected.

• Reactivity: Hu, Mu, Rt
• Applications: IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

EAAT3 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: FGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQ
• Predicted Species: Mouse (97%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC1A1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for EAAT3 Antibody

• EAAC1
• EAAC1excitatory amino acid carrier 1
• EAAT3
• EAAT3excitatory amino acid transporter 3
• Excitatory amino-acid carrier 1
• Neuronal and epithelial glutamate transporter
• SLC1A1
• Sodium-dependent glutamate/aspartate transporter 3
• solute carrier family 1 (neuronal/epithelial high affinity glutamatetransporter, system Xag), member 1
• Solute carrier family 1 member 1

Background

The EAAT3 gene encodes for glutamate transporters (also known as excitatory amino acid transporters, EAATs), that rapidly move glutamate from the postsynaptic cleft through the plasma membrane. The proteins are approximately 542 amino acids long at a mass barely over 57 kDA. This function allows for neurotoxic levels of glutamate in the brain to be avoided. It is common to see underactivity of glutamate receptors in some disorders such as ischemia and traumatic brain injuries and overactivity to be linked to mental illnesses such as schizophrenia. EAAT3 gene is also related to obsessive-compulsive disorder, ganglioglioma, autism spectrum disorders, aminoaciduriam temporal lobe epilepsy, hepatic encephalopathy, neuronitis, anorexia nervosa, and amyotrophic lateral sclerosis. It interacts with KNCN, HCCS, EWSR1, PRKCA, and ARL6IP5 to function in pathways of glutamic acid signaling, transmembrane transport of small molecules, and protein digestion and absorption.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC1A1