| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59319-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29747-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1869SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05771SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6048-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84940-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84939-25ul | |||
![Immunohistochemistry: EAAT3 Antibody (4D6.2) - BSA Free [NBP2-29747] - Mouse anti-Glutamate Transporter [EAAC1] staining of rat cerebral cortex.](http://images.novusbio.com/fullsize/EAAT3-Antibody-4D6-2-BSA-Free-Immunohistochemistry-NBP2-29747-img0002.jpg "Immunohistochemistry: EAAT3 Antibody (4D6.2) - BSA Free [NBP2-29747] - Mouse anti-Glutamate Transporter [EAAC1] staining of rat cerebral cortex.")
![ELISA: Rat EAAT3 ELISA Kit (Colorimetric) [NBP2-82471] - Samples were spiked with high concentrations of Rat EAAT3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82471_rat-eaat3-elisa-kit-colorimetric-132202416173451.jpg "ELISA: Rat EAAT3 ELISA Kit (Colorimetric) [NBP2-82471] - Samples were spiked with high concentrations of Rat EAAT3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat EAAT3 ELISA Kit (Colorimetric) [NBP2-82471] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82471_rat-eaat3-elisa-kit-colorimetric-432024858258.jpg)
![Western Blot: EAAT3 Antibody [NBP1-62519] - Titration: 0.2-1 ug/ml, Positive Control: 721_B cell lysate.](http://images.novusbio.com/fullsize/EAAT3-Antibody-Western-Blot-NBP1-62519-img0003.jpg "Western Blot: EAAT3 Antibody [NBP1-62519] - Titration: 0.2-1 ug/ml, Positive Control: 721_B cell lysate.")
![Western Blot: EAAT3 Antibody [NBP1-59880] - PANC1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/EAAT3-Antibody-Western-Blot-NBP1-59880-img0002.jpg "Western Blot: EAAT3 Antibody [NBP1-59880] - PANC1 cell lysate, concentration 0.2-1 ug/ml.")
![Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Intracellular staining of HEK293 cells (1 x 10^6 cells/mL) with SLC1A3 antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Intracellular-NB100-1869-img0011.jpg "Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Intracellular staining of HEK293 cells (1 x 10^6 cells/mL) with SLC1A3 antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).")
![Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of SLC1A3 in rat cerebellum sections](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-NB100-1869-img0005.jpg)
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 in 1. Human brain 2. Mouse brain and 3. Rat brain whole cell lysates.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Western-Blot-NB100-1869-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - SLC1A3 antibody was tested at 1:250 in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1869-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Human astrocytes express MHC-II in brain tissue from PD patients and healthy controls. The aSYN pathology was confirmed in the PD cases by immunostaining of aSYN and phosphorylated aSYN (p-aSYN). Astrocytes labeled with EAAT1/GLAST-1/SLC1A3 are expressing high levels of MHC-II, as well as Iba1-labeled microglia. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-020-01776-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1869-img0025.jpg)
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 on mouse brain.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NB100-1869-img0006.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - An intracellular stain was performed on rat FR cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody---BSA-Free-Flow-Cytometry-NB100-1869-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Human astrocytes express MHC-II in brain tissue from PD patients and healthy controls. The aSYN pathology was confirmed in the PD cases by immunostaining of aSYN and phosphorylated aSYN (p-aSYN). Astrocytes labeled with EAAT1/GLAST-1/SLC1A3 are expressing high levels of MHC-II, as well as Iba1-labeled microglia. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-020-01776-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1869-img0024.jpg)
![Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of HEK293 cells (1 x 10^6 cells/mL) with AF488 conjugated EAAT-1 antibody (orange) stained at a dilution of 1:500. Shown with rIgG (AF488) isotype control (blue).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Intracellular-NB100-1869-img0012.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 antibody NB100-1869PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0019.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0020.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0021.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Caco-2 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0022.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Neuro2a cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0023.jpg)
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Damage response is restricted to MG. (A) RT-PCR analysis for Slc1a3, the gene encoding GLAST, at the indicated times after injury. (B) RT-PCR for MG & a photoreceptor-specific marker (Nrl) in GLAST-positive & negative fractions, after MACS in intact retinas. (C–F) qPCR quantification of Oct4, Nanog, Lin28, & Dnmt3b expression levels at the indicated time after injury in MACS GLAST-positive & negative fraction; C, intact retina as control (Student's t-test; ***p < 0.001; **p < 0.01; *p < 0.05). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnins.2016.00523/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415304223.jpg)
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Damage response is restricted to MG. (A) RT-PCR analysis for Slc1a3, the gene encoding GLAST, at the indicated times after injury. (B) RT-PCR for MG & a photoreceptor-specific marker (Nrl) in GLAST-positive & negative fractions, after MACS in intact retinas. (C–F) qPCR quantification of Oct4, Nanog, Lin28, & Dnmt3b expression levels at the indicated time after injury in MACS GLAST-positive & negative fraction; C, intact retina as control (Student's t-test; ***p < 0.001; **p < 0.01; *p < 0.05). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnins.2016.00523/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415382418.jpg)
![Simple Western: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Protein changes in ONHAs corroborate bioenergetics data. (A) Glucose transporter-1 protein levels in Stretched ONH astrocytes are significantly higher than Control (*p = 0.0225, n = 7 Control, n = 8 Stretch). Retinal lysate from a 2 month-old mouse was used as a positive control for each protein analyzed, while negative control was the signal obtained when no primary antibody was included in the capillary. (B) Lactate dehydrogenase-A, the astrocyte-specific isoform of the enzyme that catalyzes the interconversion of pyruvate & lactate, has equivalent protein levels in Control & Stretch ONH astrocytes. (C) Glucose-6-phosphate dehydrogenase, the enzyme that shunts glucose into the pentose phosphate pathway, is no different in Control & Stretch ONH astrocytes. (D) Glutamine synthetase, the enzyme that synthesizes glutamine from glutamate, is no different in Control & Stretch ONH astrocytes. (E) The monomeric form of glutamate-aspartate transporter (GLAST) has significantly higher protein levels in the Stretch as compared to the Control ONH astrocytes (p = 0.020; n = 4 Control, n = 5 Stretch). (F) GLAST dimer protein levels are no different in Control & Stretch ONH astrocytes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35992925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-31020241553387.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Depletion of microglia promotes astrocyte responses following MPTP treatment. C57BL/6 mice received PLX3397 or vehicle for 21 d prior to saline or MPTP administration (intraperitoneal injection). Mice continued to receive PLX3397 or vehicle until the experiments ended. At d 7 after saline or MPTP injection, single cell suspensions were prepared from substantia nigra & striatal tissues. A) Flow cytometry plots show the gating strategy for astrocytes (GLAST+) & their expression of TNF-alpha , IL-6, iNOS, IP-10, CCL2, & CCL21. B, C) Bar graphs show the effects of microglial depletion on the expression of TNF-alpha , IL-6, iNOS, IP-10, CCL2, & CCL21 in astrocytes at d 7 after MPTP treatment. All data are presented as means ± sem; n = 9 mice/group. *P < 0.05, **P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29401614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415525277.jpg)
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Analysis of extracellular levels of GABA & Glutamate & membrane expression of their transporters. Extracellular levels of (A,C) GABA & glutamate were analyzed by microdialysis at 4 weeks. Membrane expression of (B) glutamate transporter GLAST & (D,E) GABA transporters GAT1 & GAT3 were analyzed using BS3 cross-linker. Values are expressed as percentage of control rats & are mean ± SEM of 12 rats per group. One-way ANOVA with Tukey’s test for GAT-1 (F(3,27) = 4.41, p < 0.05), GAT-3 (F(3,41) = 3.27, p < 0.05) & glutamate (F(3,36) = 3.00, p < 0.05) & Welch’s ANOVA with Dunnett’s test for GABA (W(3,19) = 3.67, p < 0.05) & GLAST (W(3,13) = 12.07, p < 0.001) were performed to compare all groups. Values significantly different from control rats are indicated by asterisks, from CCl4 rats by a, & from C-RIF rats by b. * p < 0.05, a p < 0.05, b p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415523910.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- EAAT1/GLAST-1/SLC1A3 Antibody NBP3-05771 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05771-img0004.jpg "Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- EAAT1/GLAST-1/SLC1A3 Antibody NBP3-05771 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of SLC1A3 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Strong cytoplasmic and membrane reactivity were observed.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP3-05771-img0001.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - An intracellular stain was performed on Hek293 cells with SLC1A3 Antibody NBP3-05771 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NBP3-05771-img0003.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NBP3-05771 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NBP3-05771-img0002.jpg)
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SLC1A3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84940-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SLC1A3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940] - Staining of human cerebral cortex, kidney, skeletal muscle and thyroid gland using Anti-PDIA3 antibody (A) NBP1-84940 shows similar protein distribution across tissues to independent antibody NBP1-84939 (B).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84940-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84940-img0012.jpg)
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84940-img0018.jpg)
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940] - Staining of human liver shows no positivity in hepatocytes.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84940-img0019.jpg)
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940] - Staining of human lymph node shows no positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84940-img0020.jpg)
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84940] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84940-img0021.jpg)
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Western blot analysis of various lysates using EAAT1/GLAST-1/SLC1A3 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16865_rabbit-eaat1-glast-1-slc1a3-mab-7y4u5-52202511402769.jpg "Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Western blot analysis of various lysates using EAAT1/GLAST-1/SLC1A3 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.")
![Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using EAAT1/GLAST-1/SLC1A3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16865_rabbit-eaat1-glast-1-slc1a3-mab-7y4u5-5220251139228.jpg)
![Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using EAAT1/GLAST-1/SLC1A3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16865_rabbit-eaat1-glast-1-slc1a3-mab-7y4u5-5220251215452.jpg)
![Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Confocal imaging of paraffin-embedded Rat brain tissue using EAAT1/GLAST-1/SLC1A3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16865_rabbit-eaat1-glast-1-slc1a3-mab-7y4u5-52202511402755.jpg)
![Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody (7Y4U5) [NBP3-16865] - Confocal imaging of paraffin-embedded Mouse brain tissue using EAAT1/GLAST-1/SLC1A3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16865_rabbit-eaat1-glast-1-slc1a3-mab-7y4u5-52202511411464.jpg)
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84939] - Staining of human pancreas shows no positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84939-img0015.jpg "Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84939] - Staining of human pancreas shows no positivity in exocrine glandular cells.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84939] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SLC1A3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84939-img0011.jpg)
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84939] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84939-img0012.jpg)
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84939] - Staining of human liver shows very weak positivity in hepatocytes.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84939-img0013.jpg)
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84939] - Staining of human lymph node shows no positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84939-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP1-84939] - Staining of human cerebral cortex, liver, lymph node and pancreas using Anti-SLC1A3 antibody (A) NBP1-84939 shows similar protein distribution across tissues to independent antibody NBP1-84940 (B).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84939-img0016.jpg)