EAAT1/GLAST-1/SLC1A3 Antibody

Product Discontinued

EAAT1/GLAST-1/SLC1A3 Antibody Summary

• Immunogen: A synthetic peptide from extracellular domain of human Excitatory amino acid transporter 1 (EAAT1, GLAST, GLAST1) conjugated to an immunogenic carrier protein was used as the immunogen.
• Specificity: EAAT1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC1A3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:300-1:2000

Reactivity Notes

Human. The antigen shares 95% identity with rat and mouse sequence.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for EAAT1/GLAST-1/SLC1A3 Antibody

• EA6
• EA6FLJ25094
• EAAT1
• EAAT1GLAST-1
• excitatory amino acid transporter 1
• GLAST1
• GLAST-1
• GLASTGLAST1
• SLC1A3
• Sodium-dependent glutamate/aspartate transporter 1
• solute carrier family 1 (glial high affinity glutamate transporter), member 3
• Solute carrier family 1 member 3

Background

FUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6). EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.; TISSUE SPECIFICITY: Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EAAT1/GLAST-1/SLC1A3 Antibody (NBP1-20135)(2)

Publications using NBP1-20135

• Serio A, Bilican B, Barmada SJ et al. Astrocyte pathology and the absence of non-cell autonomy in an induced pluripotent stem cell model of TDP-43 proteinopathy. Proc Natl Acad Sci U S A 2013-02-11 [PMID: 23401527] (ICC/IF, Human)
• Gupta K, Patani R, Baxter P et al. Human embryonic stem cell derived astrocytes mediate non-cell-autonomous neuroprotection through endogenous and drug-induced mechanisms. Cell Death and Differentiation. 2011-11-18 [PMID: 22095276]

Review for EAAT1/GLAST-1/SLC1A3 Antibody (NBP1-20135) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Hongxia Hu WB Human 12/15/2011

Summary

• Application: Western Blot
• Sample Tested: RCC4 Whole cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: SLC1A3
• Entrez:
  • 6507(Human)
  • 20512(Mouse)
  • 29483(Rat)