EAAT1/GLAST-1/SLC1A3 Antibody (482420) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: IHC
• Clone: 482420
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

EAAT1/GLAST-1/SLC1A3 Antibody (482420) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human EAAT1/GLAST‑1
  His146-Ser237
  Accession # P43003
• Specificity: Detects human EAAT1/GLAST-1 in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EAAT1/GLAST-1/SLC1A3 Antibody (482420) [Alexa Fluor® 594]

• EA6
• EA6FLJ25094
• EAAT1
• EAAT1GLAST-1
• excitatory amino acid transporter 1
• GLAST1
• GLAST-1
• GLASTGLAST1
• SLC1A3
• Sodium-dependent glutamate/aspartate transporter 1
• solute carrier family 1 (glial high affinity glutamate transporter), member 3
• Solute carrier family 1 member 3

Background

GLAST-1 (Sodium dependent GLu/ASp Transporter 1; also SLC1A3 and EAAT1) is a 60‑62 kDa member of the SDF symporter family of molecules. It is expressed by glia, fibroblasts, and select neuron cell types such as hippocampal neurons. EAAT1 is known to transport L‑glutamate into glia, thus removing glutamate from synaptic areas where it either acts too long, or becomes toxic at elevated concentration. Its action is dependent on its ability to cotransport sodium. Human EAAT1 is a 542 amino acid (aa), 8-transmembrane variably glycosylated protein that contains N- and C-terminal cytoplasmic domains. Homodimers are reported to exist, but only when EAAT1 shows glycosylation. There are at least two potential isoform variants. One shows an Arg substitution for aa 430‑475, while a second shows a four aa substitution for aa 62‑542. Over an extracellular loop that encompasses aa 146‑237, human EAAT1 shares 92% aa identity with mouse EAAT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.