E6AP/UBE3A Antibody (1077315)

E6AP/UBE3A was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11546) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

E6AP/UBE3A was detected in fixed A172 human glioblastoma cell line using Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11546) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 1077315
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

E6AP/UBE3A Antibody (1077315) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human E6AP/UBE3A
  Met1-Leu875
  Accession # Q05086
• Specificity: Detects recombinant human His6-E6AP/UBE3A in Direct ELISA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E6AP/UBE3A Antibody (1077315)

• ANCR
• ANCREPVE6AP
• AS
• CTCL tumor antigen se37-2
• E6AP ubiquitin-protein ligase
• E6AP
• E6-AP
• EC 6.3.2.-
• EPVE6AP
• FLJ26981
• HPVE6A
• human papilloma virus E6-associated protein
• Human papillomavirus E6-associated protein
• Oncogenic protein-associated protein E6-AP
• Renal carcinoma antigen NY-REN-54
• UBE3A
• ubiquitin protein ligase E3A
• ubiquitin-protein ligase E3A

Background

Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).

1. Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343.
2. Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156.
3. Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095.
4. Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765.
5. Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407.
6. Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q05086(Human)