E4F1 Antibody (PCRP-E4F1-2D1)

Images

 
Immunocytochemistry/ Immunofluorescence: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269] - Immunofluorescence Analysis of PFA-fixed MCF7 cells. E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1) followed by goat anti- Mouse ...read more
Flow Cytometry: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269] - Flow Cytometric Analysis of PFA-fixed HeLa cells. E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1) followed by goat anti- Mouse IgG-CF488 (blue); unstained ...read more
Protein Array: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, PA
Clone
PCRP-E4F1-2D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

E4F1 Antibody (PCRP-E4F1-2D1) Summary

Immunogen
Recombinant full-length human E4F1 protein (Uniprot: Q66K89)
Localization
Nucleus.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
E4F1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Protein Array
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08349)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for E4F1 Antibody (PCRP-E4F1-2D1)

  • E4F transcription factor 1p50E4F
  • E4FMGC99614
  • EC 6.3.2.-
  • p120E4F
  • Putative E3 ubiquitin-protein ligase E4F1
  • Transcription factor E4F
  • transcription factor E4F1

Background

The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
H00009354-M08
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
NBP1-84943
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB100-79781
Species: Hu
Applications: IHC, IHC-P, IP, WB
NBP2-14119
Species: Hu
Applications: IHC, IHC-P
NBP2-45646
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP3-16628
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-92192
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB100-56340
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NB100-56598
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P, KD, WB
NBP1-51689
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-03644
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
212-GD/CF
Species: Hu
Applications: Bind, BA
NBP1-31330
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
1310-SE
Species: Hu
Applications: EnzAct
NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-99913
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP1-84978
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for E4F1 Antibody (NBP3-07269) (0)

There are no publications for E4F1 Antibody (NBP3-07269).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for E4F1 Antibody (NBP3-07269) (0)

There are no reviews for E4F1 Antibody (NBP3-07269). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for E4F1 Antibody (NBP3-07269) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional E4F1 Products

Bioinformatics Tool for E4F1 Antibody (NBP3-07269)

Discover related pathways, diseases and genes to E4F1 Antibody (NBP3-07269). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for E4F1 Antibody (NBP3-07269)

Discover more about diseases related to E4F1 Antibody (NBP3-07269).
 

Pathways for E4F1 Antibody (NBP3-07269)

View related products by pathway.

PTMs for E4F1 Antibody (NBP3-07269)

Learn more about PTMs related to E4F1 Antibody (NBP3-07269).

Blogs on E4F1

There are no specific blogs for E4F1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our E4F1 Antibody (PCRP-E4F1-2D1) and receive a gift card or discount.

Bioinformatics

Gene Symbol E4F1
Uniprot