E4F1 Antibody (PCRP-E4F1-2D1)

Immunocytochemistry/Immunofluorescence: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269] - Immunofluorescence Analysis of PFA-fixed MCF7 cells. E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1) followed by goat anti- Mouse IgG-CF488 (green); counterstain phalloidin (red).

Flow Cytometry: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269] - Flow Cytometric Analysis of PFA-fixed HeLa cells. E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Protein Array: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1).

• Reactivity: Hu
• Applications: Flow, ICC/IF, MA
• Clone: PCRP-E4F1-2D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

E4F1 Antibody (PCRP-E4F1-2D1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08349)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human E4F1 protein (Uniprot: Q66K89)
• Localization: Nucleus.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: E4F1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for E4F1 Antibody (PCRP-E4F1-2D1)

• E4F transcription factor 1p50E4F
• E4FMGC99614
• EC 6.3.2.-
• p120E4F
• Putative E3 ubiquitin-protein ligase E4F1
• Transcription factor E4F
• transcription factor E4F1

Background

The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: E4F1
• Uniprot:
  • Q66K89(Human)