E2F-1 Antibody (18E10) - Azide and BSA Free

Western Blot: E2F1 Antibody (18E10) [NB600-209] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: E2F-1 Antibody (18E10) [NB600-209] - E2F-1 antibody [18E10] detects E2F-1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse ovary.E2F-1 stained by E2F-1 antibody [18E10] (NB600-209) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: 18E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

E2F-1 Antibody (18E10) - Azide and BSA Free Summary

• Immunogen: The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: E2F1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation
  • Western Blot
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for E2F-1 Antibody (18E10) - Azide and BSA Free

• E2F transcription factor 1
• E2F1
• E2F-1
• PBR3
• PRB-binding protein E2F-1
• RBAP1
• RBAP-1
• RBBP3
• RBBP-3
• RBP3
• Retinoblastoma-associated protein 1
• Retinoblastoma-binding protein 3
• transcription factor E2F1

Background

Clone 1G10 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E2F-1 Antibody (NB600-209)(2)

Publications using NB600-209

• Malhotra S, Lapointe J, Salari K et al. A tri-marker proliferation index predicts biochemical recurrence after surgery for prostate cancer. PLoS One 2011-01-01 [PMID: 21629784] (IF/IHC, Human)
• Liu X, Clements A, Zhao K et al. Structure of the human Papillomavirus E7 oncoprotein and its mechanism for inactivation of the retinoblastoma tumor suppressor. J Biol Chem 2006-01-01 [PMID: 16249186] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: E2F1
• Entrez:
  • 1869(Human)
• Uniprot:
  • Q01094()