Western Blot: E2F-1 Antibody (16G7) [NB600-210] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [16G7] diluted at 1:1000. The signal was developed with ...read more
Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (16G7) [NB600-210] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E2F1 protein stained by E2F1 antibody [16G7] diluted at 1:200. Red: ...read more
47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Protein G purified
Alternate Names for E2F-1 Antibody (16G7)
E2F transcription factor 1
PRB-binding protein E2F-1
Retinoblastoma-associated protein 1
Retinoblastoma-binding protein 3
transcription factor E2F1
Clone 16G7 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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