E2F-1 Antibody (16G7)

Images

 
Western Blot: E2F-1 Antibody (16G7) [NB600-210] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [16G7] diluted at 1:1000. The signal was developed with ...read more
Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (16G7) [NB600-210] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E2F1 protein stained by E2F1 antibody [16G7] diluted at 1:200. Red: ...read more
Immunohistochemistry-Paraffin: E2F-1 Antibody (16G7) [NB600-210] - Paraffin-embedded mouse cervix. E2F1 antibody [16G7] diluted at 1:500.
Immunohistochemistry-Paraffin: E2F-1 Antibody (16G7) [NB600-210] - Analysis of paraffin-embedded HBL435 xenograft, antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: E2F-1 Antibody (16G7) [NB600-210] - Analysis of paraffin-embedded Mouse prostate, antibody at 1:150 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
16G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

E2F-1 Antibody (16G7) Summary

Immunogen
The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.
Specificity
This is specific for human E2F-1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
E2F1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Immunoprecipitation
  • Western Blot 1:500 - 1:3000
Application Notes
IP-Assay dependent
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB600-210 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for E2F-1 Antibody (16G7)

  • E2F transcription factor 1
  • E2F1
  • E2F-1
  • PBR3
  • PRB-binding protein E2F-1
  • RBAP1
  • RBAP-1
  • RBBP3
  • RBBP-3
  • RBP3
  • Retinoblastoma-associated protein 1
  • Retinoblastoma-binding protein 3
  • transcription factor E2F1

Background

Clone 16G7 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E2F-1 Antibody (NB600-210)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol E2F1
Entrez