Human E-Selectin/CD62E ELISA Kit (Colorimetric)

ELISA: Human E-Selectin/CD62E ELISA Kit (Colorimetric) [NBP1-84820] - Representative standard curve for human sE-selectin ELISA. Human sE-selectin was diluted in serial 2-fold steps inSample Diluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

Product Discontinued

Human E-Selectin/CD62E ELISA Kit (Colorimetric) Summary

• Specificity: This antibody targets the soluble protein. The assay detects both natural and recombinant human sE-selectin. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human sEselectin positive serum. There was no crossreactivity detected, namely not with IL-8, ICAM-1, TNF-R, TNF beta, CD8, IL-2R, IL-6, Lselectin and P-selectin.
• Standard Curve Range: 0.8 - 50 ng/mL
• Sensitivity: 0.3 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.0%
• Intra-Assay: CV% < 5.4%
• Spike Recovery: The amount of endogenous human sE-selectin in unspiked serum was subtracted from the spike values. The recovery ranged from 71% to 95% with an overall mean recovery of 86%.
• Sample Volume: 20 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SELE

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human E-Selectin/CD62E ELISA Kit (Colorimetric)

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

The human sE-selectin ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sE-selectin. Endothelial Leukocyte Adhesion Molecule-1 belongs to the selectin family of adhesion molecules. Together with L-selectin and P-selectin, E-selectin mediates the initial interactions of leukocytes and platelets with endothelial cells. Molecular structure: The extracellular part of all selectins consists of an aminoterminal c-type lectin domain which specifically binds to carbohydrate ligands. This is followed by an EGF-like domain, and, in the case of E-selectin, by 6 short consensus repeats. The transmembrane portion of the molecule is followed by a short cytoplasmic tail. Selectins guide non-activated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer. Together with P-selectin, E-selectin is expressed on cytokine-activated endothelial cells, and contributes to the adhesion of still resting leukocytes to the endothelium. This initial binding event is an essential prerequisite for the activation of the immune cells via different inflammatory mediators. In contrast to P-selectin, E-selectin is maximally expressed 2-4 hours after cell activation. Within the next 24-48 hours E-selectin is again eliminated from the cytoplasmic membrane by shedding into the circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SELE