Mouse Dystrophin ELISA Kit (Chemiluminescence)

Standard Curve Reference.

Samples were spiked with high concentrations of Mouse Dystrophin, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL

Mouse Dystrophin ELISA Kit (Chemiluminescence) Summary

• Description: Assay Length: 3 h 30 min
• Specificity: This kit recognizes Mouse Dystrophin in samples. No significant cross-reactivity or interference between Mouse Dystrophin and analogues was observed
• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10
• Intra-Assay: CV% < 10
• Spike Recovery: 80 - 120%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: DMD

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Dystrophin ELISA Kit (Chemiluminescence)

• BMDDXS272
• CMD3B
• DXS142
• DXS164
• DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272
• DXS206
• DXS230
• DXS239
• DXS268
• DXS269
• DXS270
• dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142
• dystrophin

Background

Dystrophin is a muscle membrane protein (427 kDa) which is absent, reduced or altered as a result of mutation in Duchenne and Becker muscular dystrophies (DMD/BMD) or its homologue in the mouse.8 Severe DMD is associated with a marked dystrophin deficiency whereas patients with the milder form of BMD show less pronounced abnormalities of protein expression. Because abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases. Predictions from the sequence suggest a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like triple-helical domain separating an N-terminal actin-binding domain from two C-terminal domains, one of which is rich in cysteine.9 The large size of dystrophin and its low abundance (<0.01% of the total muscle protein) are a hindrance to the isolation of intact, native protein for structure/function studies. Monoclonal antibodies against defined regions10 of dystrophin provide a means for studying its structure and function, interactions with other proteins and the nature of the partial gene products produced in some patients carrying deletions in the dystrophin gene. The antibodies are useful in the prenatal or post-abortion diagnosis of muscular dystrophy carriers by immunohistological analyses.11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Dystrophin ELISA Kit (NBP3-49692). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: DMD