Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

Western blot shows lysates of human placenta tissue and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Dynactin Subunit 2/DCTN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6850) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dynactin Subunit 2/ DCTN2 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Dynactin Subunit 2/DCTN2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Dynactin Subunit 2/DCTN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6850) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Dynactin Subunit 2/DCTN2/DCTN-50 Antibody Summary

• Immunogen: E. coli-derived recombinant human Dynactin Subunit 2/DCTN2
  Asp277-Lys401
  Accession # Q13561
• Specificity: Detects human Dynactin Subunit 2/DCTN2 in direct ELISAs and human and mouse Dynactin Subunit 2/DCTN2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DCTN1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DCTN2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

• DCTN2
• DCTN-50
• DCTN5050 kD dynein-associated polypeptide
• dynactin 2 (p50)
• dynactin complex 50 kD subunit
• Dynactin complex 50 kDa subunit
• Dynactin Subunit 2
• dynactin subunit 2,50 kDa dynein-associated polypeptide
• Dynamitin
• p50 dynamitin
• RBP50

Background

DCTN-2 (Dynactin-2; also 50 kDa dynein-associated polypeptide and p50 dynamitin) is a 50 kDa intracellular member of the dynactin subunit 2 family of phosphoproteins. It is ubiquitously expressed and serves to stabilize the dynactin complex of 11 different proteins. This complex is an obligate cofactor of dynein and kinesin motors, which position the mitotic spindle for cell division, and move vesicles within the cell. Human DCTN-2 is 401 amino acids (aa) in length. It contains three coiled‑coil regions (aa 99-132, 214-244 and 379-399), two potential phosphorylation sites (Ser83 and Tyr86), and one acetylation site at Ala2. The first two coiled‑coil regions contribute to oligomerization, while aa 1-91 are essential for dynactin regulation. Potential isoform variants utilize either a five or two aa insertion after Ala35, an alternative start site at Met88, and a 21 aa substitution for aa 118-401. Over aa 277-401, human DCTN-2 shares 95% aa identity with mouse DCTN-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCTN2
• Uniprot:
  • Q13561(Human)