Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (696206) [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Dynactin Subunit 2/DCTN2 Monoclonal Antibody (Catalog # MAB6850) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Dynactin Subunit 2/DCTN2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Dynactin Subunit 2/DCTN2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Dynactin Subunit 2/DCTN2 Monoclonal Antibody (Catalog # MAB6850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue. Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 696206
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (696206) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Dynactin
  Subunit 2/DCTN2
  Asp277-Lys401
  Accession # Q13561
• Specificity: Detects human Dynactin Subunit 2/DCTN2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human
  Dynactin Subunit 1/DCTN1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DCTN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (696206) [Unconjugated]

• DCTN2
• DCTN-50
• DCTN5050 kD dynein-associated polypeptide
• dynactin 2 (p50)
• dynactin complex 50 kD subunit
• Dynactin complex 50 kDa subunit
• Dynactin Subunit 2
• dynactin subunit 2,50 kDa dynein-associated polypeptide
• Dynamitin
• p50 dynamitin
• RBP50

Background

DCTN2 (Dynactin-2; also 50 kDa Dynein-associated polypeptide and p50 Dynamitin) is a 50 kDa intracellular member of the Dynactin family of phosphoproteins. It is ubiquitously expressed and serves to stabilize the Dynactin complex of 11 different proteins. This complex is an obligate cofactor of Dynein and Kinesin motors, which position the mitotic spindle for cell division and move vesicles within the cell. Human Dynactin Subunit 2 is 401 amino acids (aa) in length. It contains three coiled‑coil regions (aa 99-132, 214-244 and 379-399), two potential phosphorylation sites (Ser83 and Tyr86), and one acetylation site at Ala2. The first two coiled‑coil regions contribute to oligomerization, while aa 1-91 are essential for Dynactin regulation. Potential isoform variants utilize either a five or two aa insertion after Ala35, an alternative start site at Met88, and a 21 aa substitution for aa 118-401. Over aa 277-401, human Dynactin Subunit 2 shares 95% aa identity with mouse Dynactin Subunit 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCTN2
• Uniprot:
  • Q13561(Human)