Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (696206) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 696206
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (696206) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Dynactin
  Subunit 2/DCTN2
  Asp277-Lys401
  Accession # Q13561
• Specificity: Detects human Dynactin Subunit 2/DCTN2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human
  Dynactin Subunit 1/DCTN1 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (696206) [Alexa Fluor® 350]

• DCTN2
• DCTN-50
• DCTN5050 kD dynein-associated polypeptide
• dynactin 2 (p50)
• dynactin complex 50 kD subunit
• Dynactin complex 50 kDa subunit
• Dynactin Subunit 2
• dynactin subunit 2,50 kDa dynein-associated polypeptide
• Dynamitin
• p50 dynamitin
• RBP50

Background

DCTN2 (Dynactin-2; also 50 kDa Dynein-associated polypeptide and p50 Dynamitin) is a 50 kDa intracellular member of the Dynactin family of phosphoproteins. It is ubiquitously expressed and serves to stabilize the Dynactin complex of 11 different proteins. This complex is an obligate cofactor of Dynein and Kinesin motors, which position the mitotic spindle for cell division and move vesicles within the cell. Human Dynactin Subunit 2 is 401 amino acids (aa) in length. It contains three coiled‑coil regions (aa 99-132, 214-244 and 379-399), two potential phosphorylation sites (Ser83 and Tyr86), and one acetylation site at Ala2. The first two coiled‑coil regions contribute to oligomerization, while aa 1-91 are essential for Dynactin regulation. Potential isoform variants utilize either a five or two aa insertion after Ala35, an alternative start site at Met88, and a 21 aa substitution for aa 118-401. Over aa 277-401, human Dynactin Subunit 2 shares 95% aa identity with mouse Dynactin Subunit 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.