Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) - Azide and BSA Free

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) [H00010540-M01] - DCTN2 monoclonal antibody (M01), clone 2G7 Analysis of DCTN2 expression in MCF-7.

Immunocytochemistry/Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) [H00010540-M01] - Analysis of monoclonal antibody to DCTN2 on MCF-7 cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) [H00010540-M01] - Detection limit for recombinant GST tagged DCTN2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) - Azide and BSA Free Summary

• Immunogen: DCTN2 (AAH00718, 302 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK
• Specificity: DCTN2 - dynactin 2 (p50)
• Isotype: IgG1 Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: DCTN2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) - Azide and BSA Free

• DCTN2
• DCTN-50
• DCTN5050 kD dynein-associated polypeptide
• dynactin 2 (p50)
• dynactin complex 50 kD subunit
• Dynactin complex 50 kDa subunit
• Dynactin Subunit 2
• dynactin subunit 2,50 kDa dynein-associated polypeptide
• Dynamitin
• p50 dynamitin
• RBP50

Background

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (H00010540-M01)(1)

Publication using H00010540-M01

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: DCTN2
• Entrez:
  • 10540(Human)
• OMIM:
  • 607376(Human)
• Uniprot:
  • AAH00718(Human)