Dynactin Subunit 1/DCTN1 Antibody (705007) [Janelia Fluor® 525]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 705007
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

Dynactin Subunit 1/DCTN1 Antibody (705007) [Janelia Fluor® 525] Summary

• Immunogen: E. coli-derived recombinant human Dynactin Subunit 1/DCTN1
  Ala1145-Ser1278
  Accession # Q14203
• Specificity: Detects human Dynactin Subunit 1/DCTN1 in direct ELISAs, and huamn and mouse DCTN1 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DCTN2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DCTN1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Dynactin Subunit 1/DCTN1 Antibody (705007) [Janelia Fluor® 525]

• DAP-150
• DCTN1
• DP-150
• dynactin 1 (p150, Glued (Drosophila) homolog)
• Dynactin 1
• Dynactin Subunit 1
• glued homolog, Drosophila)
• HMN7B
• p135
• p150 Glued (Drosophila) homolog
• p150-glued

Background

The DCTN1 gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCTN1