DTX2 Antibody

Immunohistochemistry-Paraffin: DTX2 Antibody [NBP1-52144] - 5ug/ml staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Rt, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DTX2 Antibody Summary

• Description: Novus Biologicals Goat DTX2 Antibody (NBP1-52144) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-DTX2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EKLSTASGYSDVTD corresponding to internal region according to NP_065943.2, NP_001096066.1.
• Epitope: C-EKLSTASGYSDVTD
• Specificity: This antibody is expected to recognize both reported isoforms (NP_065943.2; NP_001096066.1). Reported variants NP_001096065.1, NP_065943.2, NP_001096064.1 represent identical protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DTX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.3ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Western Blot detection limit 1:32000
• Application Notes: Western blot: Preliminary experiments gave an approx 85kDa band in lysates of cell lines A549, HepG2 and NIH3T3 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.2kDa according to NP_065943.2. The 85kDa band was successfully blocked by incubation with the immunizing peptide
  IHC: Paraffin embedded Human Small Intestine.

Reactivity Notes

Expected from sequence similarity: Rat, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DTX2 Antibody

• deltex (Drosophila) homolog 2
• deltex homolog 2 (Drosophila)
• Deltex2
• hDTX2
• MGC71098
• protein deltex-2
• RING finger protein 58
• RNF58KIAA1528deltex2
• zinc ion binding protein

Background

DTX2 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 (PubMed 12670957)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DTX2 Antibody (NBP1-52144)(1)

Publication using NBP1-52144

• Yi Z, Yi T, Wu Z. cDNA cloning, characterization and expression analysis of DTX2, a human WWE and RING-finger gene, in human embryos. DNA Res 1996-02-29 [PMID: 17286044]

Bioinformatics

• Gene Symbol: DTX2
• Entrez:
  • 113878(Human)
  • 304591(Rat)
• Uniprot:
  • NP_065943.2(Human)