DSCAM Antibody (399212) [Unconjugated] - Long Isoform

Western blot shows 25 ng of Recombinant Human DSCAM (Catalog # 3666-DS) and Recombinant Human DSCAM-L1 (Catalog # 3315-DL). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human DSCAM Long Isoform Monoclonal Antibody (Catalog # MAB36661) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DSCAM at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

DSCAM was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human DSCAM Long Isoform Monoclonal Antibody (Catalog # MAB36661) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (orange; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

The C-terminus of DSCAM is not required for protein stability or localization. (C) Western blots of DSCAM protein immunoprecipitated from neonatal brains showed no change in the size or amount of DSCAM in Dscam∆C/∆C mutants. The antibody specificity is confirmed by the lack of signal from Dscam-/- brains. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27637097), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 399212
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DSCAM Antibody (399212) [Unconjugated] - Long Isoform Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DSCAM Long Isoform
  Glu18-Met1595
  Accession # O60469
• Specificity: Detects human DSCAM Long Isoform in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human DSCAM-L1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DSCAM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DSCAM Antibody (399212) [Unconjugated] - Long Isoform

• CHD2-42
• CHD2-52
• Down syndrome cell adhesion molecule
• DSCAM
• human CHD2-52 down syndrome cell adhesion molecule, 10CHD2

Background

Down syndrome cell adhesion molecule (DSCAM) is a 220 kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily (1). Human DSCAM, which maps to a Down syndrome region of chromosome 21q22.2‑22.3, is synthesized as a 2012 amino acid (aa) precursor that contains a 17 aa signal sequence, a 1578 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 396 aa cytoplasmic tail. The ECD contains ten Ig-like C2-type domains, six fibronectin type III domains, and 16 potential sites for N-linked glycosylation. Splicing variants lead to a second, shorter isoform, which has a ten aa substitution for aa 1562‑1571 in the longer isoform, and a deletion of residues corresponding to aa 1572‑2012 in the longer isoform. Human mature DSCAM is 98% aa identical to mature mouse and rat DSCAM. Studies on mice have shown that DSCAM is expressed widely in the developing nervous system (1, 2). More recent studies indicate that DSCAM plays an important role in neurite arborization, cell body spacing, and lamina-specific synaptic targeting in vertebrate retina (2‑4). DSCAM directly binds to cytoplasmic Pak1 and stimulates Pak1 phosphorylation and activity (5). In addition, DSCAM activates both JNK and p38 MAP kinases, and expression of the cytoplasmic domain of DSCAM induces a morphological change in cultured cells that is JNK-dependent (5). Thus, it appears that DSCAM signals through Pak1 and functions in axon guidance. Furthermore, DSCAM, in collaboration with DCC, interacts with Netrin-1 and is a receptor required for Netrin-dependent commissural axon outgrowth and pathfinding (2, 6).

1. Yamakawa, K. et al. (1998) Hum. Mol. Genet. 7:227.
2. Liu, G. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:2951.
3. Fuerst, P.G. et al. (2008) Nature 451:470.
4. Yamagata, M. and J.R. Sanes (2008) Nature 451:465.
5. Li, W. and K.-L. Guan (2004) J. Biol. Chem. 279:32824.
6. Ly, A. et al. (2008) Cell 133:11241.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DSCAM Antibody (MAB36661)(2)

Publications using MAB36661

• Replacing the PDZ-interacting C-termini of DSCAM and DSCAML1 with epitope tags causes different phenotypic severity in different cell populations Elife, 2016-09-16;5(0):. 2016-09-16 [PMID: 27637097] (Immunoprecipitation, Mouse)
• de Andrade GB, Long SS, Fleming H et al. DSCAM localization and function at the mouse cone synapse. J Comp. Neurol. 1/30/2014 [PMID: 24477985]
  
  Details:
  Neurokinin B Receptor antibody used at 1:2000 dilution for IHC-Fr staining of type 1 and 2 cone bipolar cells in mice. Figure 2 A-D - adult wild-type and Dscam2J/2J retina section. Figure 14 O - sections of DscamFD/FD retina.

Bioinformatics

• Gene Symbol: DSCAM
• Uniprot:
  • O60469()