DSCAM Antibody (399212) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 399212
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

DSCAM Antibody (399212) [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DSCAM Long Isoform
  Glu18-Met1595
  Accession # O60469
• Specificity: Detects human DSCAM Long Isoform in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human DSCAM-L1 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DSCAM Antibody (399212) [Alexa Fluor® 594]

• CHD2-42
• CHD2-52
• Down syndrome cell adhesion molecule
• DSCAM
• human CHD2-52 down syndrome cell adhesion molecule, 10CHD2

Background

Down syndrome cell adhesion molecule (DSCAM) is a 220 kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily (1). Human DSCAM, which maps to a Down syndrome region of chromosome 21q22.2‑22.3, is synthesized as a 2012 amino acid (aa) precursor that contains a 17 aa signal sequence, a 1578 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 396 aa cytoplasmic tail. The ECD contains ten Ig-like C2-type domains, six fibronectin type III domains, and 16 potential sites for N-linked glycosylation. Splicing variants lead to a second, shorter isoform, which has a ten aa substitution for aa 1562‑1571 in the longer isoform, and a deletion of residues corresponding to aa 1572‑2012 in the longer isoform. Human mature DSCAM is 98% aa identical to mature mouse and rat DSCAM. Studies on mice have shown that DSCAM is expressed widely in the developing nervous system (1, 2). More recent studies indicate that DSCAM plays an important role in neurite arborization, cell body spacing, and lamina-specific synaptic targeting in vertebrate retina (2‑4). DSCAM directly binds to cytoplasmic Pak1 and stimulates Pak1 phosphorylation and activity (5). In addition, DSCAM activates both JNK and p38 MAP kinases, and expression of the cytoplasmic domain of DSCAM induces a morphological change in cultured cells that is JNK-dependent (5). Thus, it appears that DSCAM signals through Pak1 and functions in axon guidance. Furthermore, DSCAM, in collaboration with DCC, interacts with Netrin-1 and is a receptor required for Netrin-dependent commissural axon outgrowth and pathfinding (2, 6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.