DPPA2 Overexpression Lysate

Product Discontinued

DPPA2 Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC205441 and based on accession number NM_138815. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens developmental pluripotency associated 2 (DPPA2), mRNA.
• Gene: DPPA2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 33.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DPPA2 Overexpression Lysate

• CT100
• developmental pluripotency associated 2
• developmental pluripotency-associated protein 2
• DPPA2
• ECSA
• PESCRG1
• PESCRG1cancer/testis antigen 100
• Pluripotent embryonic stem cell-related gene 1 protein

Background

DPPA2, also known as Developmental pluripotency-associated protein 2, is a 33.8 kDa, 298 amino acid protein that is involved in binding to target gene promoters, such as SYCE1 and NKX2-5. Diseases such as epithelial ovarian cancer and lung cancer have been researched with this protein. The protein interacts with other proteins such as DPPA4, FXR2, MCM10, DVL2, and AP2M1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: DPPA2