DPPA2 Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

DPPA2 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human DPPA2
  Ser2-Thr174
  Accession # Q7Z7J5
• Specificity: Detects human DPPA2 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPPA2 Antibody [Alexa Fluor® 532]

• CT100
• developmental pluripotency associated 2
• developmental pluripotency-associated protein 2
• DPPA2
• ECSA
• PESCRG1
• PESCRG1cancer/testis antigen 100
• Pluripotent embryonic stem cell-related gene 1 protein

Background

DPPA2 (Developmental pluripotency associated 2; also ESCA, CT100 and PESCRG1) is a 38-39 kDa member of both the DPPA and cancer-testis family of proteins. It is found in pluripotent stem cells in a pattern that parallels that of Oct4, and likely contributes to formation of the ICM, plus the maintainence of cells in an undifferentiated state. DPPA2 is also found in spermatocytes and select tumor types. Human DPPA2 is 298 amino acids (aa) in length. It contains one DNA-binding SAP domain that is involved in chromosomal organization (aa 92-126). One potential isoform variant has been reported that shows a deletion of aa 61-71. Over aa 2‑174, human DPPA2 shares only 36% aa identity with mouse DPPA2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.