Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free

Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free [NBP2-80700] - Analysis of lysates from Sf9 cells which were transfected with rat DR-D1b (Lane 1) or DR-D1a (Lane 2) using DRD1 antibody (clone SG2-D1a). This clone detected D1a receptor only and was not cross-reactive against DR-D1b (Note: these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.) Image from the standard format of this antibody.

Immunocytochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free [NBP2-80700] - Analysis of Dopamine Receptor D1 in rat retinal whole mounts and sections. Image from verified customer review. Image from the standard format of this antibody.

Immunohistochemistry Free-Floating: Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free [NBP2-80700] - Analysis of Dopamine D1 R in free floating rat brain slices using Dopamine D1 R antibody. Image from verified customer review. Image from the standard format of this antibody.

• Reactivity: Mu, Rt, Bv(-), Ca(-), Hu(-), Po(-), Rb(-), Sh(-)
• Applications: WB, ICC/IF, IHC, IHC
• Clone: SG2-D1a
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free Summary

• Immunogen: Recombinant rat Dopamine Receptor D1 protein near the C-terminus. [Swiss-Prot# P18901]
• Localization: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DRD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Western Blot 1:200 - 1:500
• Application Notes: In Western blot multiple bands are seen around 45 and 100 kDa (dimer) in SF9 cell lysates overexpressing D1a and a single band at 90 kDa in striatal tissue lysate. Do NOT boil samples.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free

• D(1A) dopamine receptor
• D1DR
• DADR
• Dopamine D1 R
• Dopamine D1 receptor
• Dopamine D1R
• dopamine receptor D1
• DRD1
• DRD1A

Background

Dopamine is a unique brain neurotransmitter which is responsible for the regulation of versatile processes such as motor activity, cognition, motivation, emotion, food intake, endocrine secretions etc. and dysfunctions in dopaminergic systems have been linked to drug addiction, schizophrenia, Parkinson's disease, Tourette's syndrome, attention-deficit and hyperactivity disorders as well as hyperprolactinemia. Dopamine Receptor D1 (DRD1), a GPCR protein, is the D1 subtype of dopamine receptors (DR) which represents the most abundant DR in CNS with capability of stimulating adenylyl cyclase and activating cyclic AMP-dependent protein kinases. DRD1 localize to cell membrane/ER membrane as multi-pass membrane protein and its transport from ER to cell surface is regulated by interaction with DNAJC14. DRD1 is highly expressed in the brain tissues, especially in the striatum, the nucleus accumbens, and the olfactory tubercle. DRD1 regulate neuronal growth and development, mediate behavioral responses, and modulate dopamine receptor D2-mediated events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DRD1