Dopamine D1R/DRD1 Antibody (SG2-D1a)

Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of lysates from Sf9 cells which were transfected with rat DR-D1b (Lane 1) or DR-D1a (Lane 2) using DRD1 antibody (clone SG2-D1a). This clone detected D1a receptor only and was not cross-reactive against DR-D1b (Note: these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.)

Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Representative images of immunofluorescence for c-fos and dopamine receptor D1 in the NAc. Image collected and cropped by CiteAb from the following publication (nature.com/articles/ncomms11829), licensed under a CC-BY license.

Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine Receptor D1 in rat retinal whole mounts and sections. Image from verified customer review.

Immunohistochemistry Free-Floating: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine D1 R in free floating rat brain slices using Dopamine D1 R antibody. Image from verified customer review.

Immunocytochemistry/ Immunofluorescence: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - NAc D1 & D2 neuronal activation predicts performance in motivation-related tasks.(a) PIT outcome (n=10). (b) Breakpoint for two PR sessions (n=10). (c) Representative immunostaining of c-fos in the NAc; Scale bar: 100 μm; inset: 20 μm. (d) Animals performing PIT or PR tests have increased c-fos+ cells in the NAc (n=6). (e) Principal factor analysis was done to evaluate the contribution of each brain region for the behavioural performance. This analysis shows that the NAc core & shell regions (NAcc & NAcs; factor 2) are grouped distinctively from other limbic regions (BLA, CeA & VTA; factor 3) & from cortical regions that are all grouped together in factor 1 (ACC, PLC, ILC, lOFC & vOFC). Representative images of immunofluorescence for c-fos & dopamine receptor D1 (f) or c-fos & dopamine receptor D2 (i) in the NAc of animals that performed PR task; Scale bar: 100 μm; inset: 20 μm. Insets represent double positive cells. (g) Number of c-fos+/D1+ cells & c-fos+/D2+ (j) cells in the NAc (n=6). (h) Correlation between individual Breakpoint & number of c-fos+/D1+ cells in the NAc (n=9). (k) Correlation between individual breakpoint & number of c-fos+/D2+ cells in the NAc (n=12). Error bars denote s.e.m. *P≤0.05, **P ≤0.01, ***P≤0.001. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms11829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Product Discontinued

Dopamine D1R/DRD1 Antibody (SG2-D1a) Summary

• Immunogen: Recombinant rat Dopamine Receptor D1 protein near the C-terminus. [Swiss-Prot# P18901]
• Localization: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
• Specificity: This antibody is specific for Dopamine Receptor D1A. There is no cross-reactivity with D1B (D5) receptor.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DRD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 31462765)
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Western Blot 1:200-1:500
• Application Notes: In Western blot multiple bands are seen around 45 and 100 kDa (dimer) in SF9 cell lysates overexpressing D1a and a single band at 90 kDa in striatal tissue lysate. Do NOT boil samples.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Dopamine D1R/DRD1 Antibody (SG2-D1a)

• D(1A) dopamine receptor
• D1DR
• DADR
• Dopamine D1 R
• Dopamine D1 receptor
• Dopamine D1R
• dopamine receptor D1
• DRD1
• DRD1A

Background

Dopamine is a unique brain neurotransmitter which is responsible for the regulation of versatile processes such as motor activity, cognition, motivation, emotion, food intake, endocrine secretions etc. and dysfunctions in dopaminergic systems have been linked to drug addiction, schizophrenia, Parkinson's disease, Tourette's syndrome, attention-deficit and hyperactivity disorders as well as hyperprolactinemia. Dopamine Receptor D1 (DRD1), a GPCR protein, is the D1 subtype of dopamine receptors (DR) which represents the most abundant DR in CNS with capability of stimulating adenylyl cyclase and activating cyclic AMP-dependent protein kinases. DRD1 localize to cell membrane/ER membrane as multi-pass membrane protein and its transport from ER to cell surface is regulated by interaction with DNAJC14. DRD1 is highly expressed in the brain tissues, especially in the striatum, the nucleus accumbens, and the olfactory tubercle. DRD1 regulate neuronal growth and development, mediate behavioral responses, and modulate dopamine receptor D2-mediated events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dopamine D1R/DRD1 Antibody (NB110-60017)(19)

Publications using NB110-60017

• He ZX, Xi K, Liu KJ et al. A Nucleus Accumbens Tac1 Neural Circuit Regulates Avoidance Responses to Aversive Stimuli International journal of molecular sciences 2023-02-22 [PMID: 36901777] (IHC-Fr, Mouse)
• Struzyna LA, Browne KD, Burrell JC et al. Axonal Tract Reconstruction Using a Tissue-Engineered Nigrostriatal Pathway in a Rat Model of Parkinson's Disease International Journal of Molecular Sciences 2022-11-12 [PMID: 36430464] (Immunohistochemistry, Western Blot, Rat)
• Shen B, Zhang R, Yang G et al. Cannabidiol prevents methamphetamine-induced neurotoxicity by modulating dopamine receptor D1-mediated calcium-dependent phosphorylation of methyl-CpG-binding protein 2 Frontiers in Pharmacology 2022-09-06 [PMID: 36147353] (Immunohistochemistry, Western Blot, Rat)
• Smith CJ, Lintz T, Clark MJ et al. Prenatal opioid exposure inhibits microglial sculpting of the dopamine system selectively in adolescent male offspring Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 2022-07-14 [PMID: 35835992] (KO, IHC-Fr, Rat)
• Kasahara Y, Masukawa D, Kobayashi K et al. L-DOPA-induced Neurogenesis in the Hippocampus is Mediated through GPR143, a Distinct Mechanism of Dopamine Stem Cells 2022-01-19 [PMID: 35257172] (IF/IHC, Mouse)
• Kisling A, Byrne S, Parekh RU Et al. Loss of Function in Dopamine D3 Receptor Attenuates Left Ventricular Cardiac Fibroblast Migration and Proliferation in vitro Frontiers in cardiovascular medicine 2021-10-11 [PMID: 34708087] (IF/IHC, ICC/IF, Mouse)
• Bourgeois JR, Kalyanasundaram G, Figueroa C et al. A semi-automated brain atlas-based analysis pipeline for c-Fos immunohistochemical data J Neurosci Methods 2020-10-20 [PMID: 33091429] (IF/IHC, Rat)
• Coimbra B, Soares-Cunha C, Vasconcelos NAP et al. Role of laterodorsal tegmentum projections to nucleus accumbens in reward-related behaviors Nat Commun 2019-09-12 [PMID: 31515512] (IF/IHC, Mouse, Rat)
• Soares-Cunha C, de Vasconcelos NAP, Coimbra B et al. Nucleus accumbens medium spiny neurons subtypes signal both reward and aversion Mol. Psychiatry 2019-08-28 [PMID: 31462765] (ICC/IF, Mouse)
• AM Kopec, CJ Smith, NR Ayre, SC Sweat, SD Bilbo Microglial dopamine receptor elimination defines sex-specific nucleus accumbens development and social behavior in adolescent rats Nat Commun, 2018-09-25;9(1):3769. 2018-09-25 [PMID: 30254300] (Immunohistochemistry, Western Blot, Rat)
• Kopec A, Smith CJ, Ayre NR et al. Microglial dopamine receptor elimination defines sex-specific nucleus accumbens development and social behavior in adolescent rats. Nat Commun 2017 Oct 29 [PMID: 30254300] (IHC-Fr, Rat)
• Kopec A, Smith CJ, Ayre NR et al. Microglial elimination of dopamine D1 receptors defines sex-specific changes in nucleus accumbens development and social play behavior during adolescence bioRxiv 2017 Oct 29 [PMID: 30254300] (IHC-Fr, Rat)
• Soares-Cunha C, Coimbra B, Domingues A et al. Nucleus Accumbens Microcircuit Underlying D2-MSN-Driven Increase in Motivation. eNeuro 2018-04-19 [PMID: 29780881] (ICC/IF, Rat)
• Morigaki R, Okita S, Goto S. Dopamine-Induced Changes in Galphaolf Protein Levels in Striatonigral and Striatopallidal Medium Spiny Neurons Underlie the Genesis of l-DOPA-Induced Dyskinesia in Parkinsonian Mice. Front Cell Neurosci. 2017-02-27 [PMID: 28239340] (IF/IHC, Mouse)
• Feng XY, Zhang DN, Wang YA et al. Dopamine enhances duodenal epithelial permeability via the dopamine D5 receptor in rodent. Acta Physiol (Oxf) 2016-10-06 [PMID: 27652590] (IF/IHC, Rat)
• Soares-Cunha C, Coimbra B, David-Pereira A et al. Activation of D2 dopamine receptor-expressing neurons in the nucleus accumbens increases motivation. Nat Commun 2016-06-24 [PMID: 27337658] (ICC/IF, Rat)
• Stradleigh TW, Ishida AT. Fixation strategies for retinal immunohistochemistry Prog Retin Eye Res 2015-04-17 [PMID: 25892361] (IF/IHC, Rat)
• Hayashida Y, Rodriguez CV, Ogata G et al. Inhibition of adult rat retinal ganglion cells by D1-type dopamine receptor activation. J Neurosci;29(47):15001-16. 2009-11-25 [PMID: 19940196] (WB, Rat)
• Luedtke, R et al. Immunoblot and immunohistochemical comparison of murine monoclonal antibodies specific for the rat D1a and D1b dopamine receptor subtypes. J Neuroimmunol;101(2):170-87. 1999-11-15 [PMID: 10580800] (WB, IF/IHC, Rat)

Reviews for Dopamine D1R/DRD1 Antibody (NB110-60017) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Rat, Other.

reviewed by: Verified Customer IF Rat 04/05/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Brain free floating slices
• Species: Rat
• Lot: A-2

reviewed by: Verified Customer ICC Other 04/10/2014

Summary

• Application: Immunocytochemistry
• Sample Tested: brain section
• Species: Other
• Lot: A-2

reviewed by: Verified Customer IHC-Fr Rat 01/18/2010

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Rat retinal whole mounts and sections
• Species: Rat
• Lot: A1

FAQs for Dopamine D1R/DRD1 Antibody (NB110-60017). (Showing 1 - 1 of 1 FAQs).

1. I am writing a manuscript and referring to results we obtained with your Dopamine Receptor D1 antibody. (These are results listed on your website: Journal of Neuroscience 29: 15001.) Your datasheet states that the immunogen was 123 residues of the rat Dopamine Receptor D1a. I realize the amino acid sequence that you used is proprietary, and do not need to know which residues you used. However, can you tell me the position of some of the amino acids so that I can state, with certainty, if these residues are not in the rat Dopamine Receptor D1b amino acid sequence?
   • I have spoken with our lab manager, and after pulling the original production data for this antibody, it does appear that the protein sequence used to synthesize the antibody was in fact the last 123 amino acids from residues 323 to 446 at the C-terminal. This updated immunogen information has been added to the datasheet to reflect that the antibody targets the C-terminus. Keep in mind the protein used contained to synthesize the antibody contained a HIS tag. I hope this information is helpful in your publication, and we are happy to hear that you are using our product for your published data.

Bioinformatics

• Gene Symbol: DRD1