Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of lysates from Sf9 cells which were transfected with rat DR-D1b (Lane 1) or DR-D1a (Lane 2) using DRD1 antibody (clone SG2-D1a). This clone ...read more
Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Representative images of immunofluorescence for c-fos and dopamine receptor D1 in the NAc. Image collected and cropped by CiteAb from the ...read more
Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine Receptor D1 in rat retinal whole mounts and sections. Image from ...read more
Immunohistochemistry Free-Floating: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine D1 R in free floating rat brain slices using Dopamine ...read more
Recombinant rat Dopamine Receptor D1 protein near the C-terminus. [Swiss-Prot# P18901]
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
This antibody is specific for Dopamine Receptor D1A. There is no cross-reactivity with D1B (D5) receptor.
Protein A purified
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In Western blot multiple bands are seen around 45 and 100 kDa (dimer) in SF9 cell lysates overexpressing D1a and a single band at 90 kDa in striatal tissue lysate. Do NOT boil samples. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:31462765).
Read 3 Reviews rated 4.7 using NB110-60017 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-Glycine, 0.15 M NaCl
0.05% Sodium Azide
Protein A purified
Alternate Names for Dopamine D1R/DRD1 Antibody (SG2-D1a)
D(1A) dopamine receptor
Dopamine D1 R
Dopamine D1 receptor
dopamine receptor D1
Dopamine is a unique brain neurotransmitter which is responsible for the regulation of versatile processes such as motor activity, cognition, motivation, emotion, food intake, endocrine secretions etc. and dysfunctions in dopaminergic systems have been linked to drug addiction, schizophrenia, Parkinson's disease, Tourette's syndrome, attention-deficit and hyperactivity disorders as well as hyperprolactinemia. Dopamine Receptor D1 (DRD1), a GPCR protein, is the D1 subtype of dopamine receptors (DR) which represents the most abundant DR in CNS with capability of stimulating adenylyl cyclase and activating cyclic AMP-dependent protein kinases. DRD1 localize to cell membrane/ER membrane as multi-pass membrane protein and its transport from ER to cell surface is regulated by interaction with DNAJC14. DRD1 is highly expressed in the brain tissues, especially in the striatum, the nucleus accumbens, and the olfactory tubercle. DRD1 regulate neuronal growth and development, mediate behavioral responses, and modulate dopamine receptor D2-mediated events.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Dopamine D1R/DRD1 Antibody (NB110-60017). (Showing 1 - 1 of 1 FAQs).
I am writing a manuscript and referring to results we obtained with your Dopamine Receptor D1 antibody. (These are results listed on your website: Journal of Neuroscience 29: 15001.) Your datasheet states that the immunogen was 123 residues of the rat Dopamine Receptor D1a. I realize the amino acid sequence that you used is proprietary, and do not need to know which residues you used. However, can you tell me the position of some of the amino acids so that I can state, with certainty, if these residues are not in the rat Dopamine Receptor D1b amino acid sequence?
I have spoken with our lab manager, and after pulling the original production data for this antibody, it does appear that the protein sequence used to synthesize the antibody was in fact the last 123 amino acids from residues 323 to 446 at the C-terminal. This updated immunogen information has been added to the datasheet to reflect that the antibody targets the C-terminus. Keep in mind the protein used contained to synthesize the antibody contained a HIS tag. I hope this information is helpful in your publication, and we are happy to hear that you are using our product for your published data.
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