Dopamine D1R/DRD1 Antibody (SG2-D1a)

Images

 
Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of lysates from Sf9 cells which were transfected with rat DR-D1b (Lane 1) or DR-D1a (Lane 2) using DRD1 antibody (clone SG2-D1a). This clone ...read more
Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Representative images of immunofluorescence for c-fos and dopamine receptor D1 in the NAc. Image collected and cropped by CiteAb from the ...read more
Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine Receptor D1 in rat retinal whole mounts and sections. Image from ...read more
Immunohistochemistry Free-Floating: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine D1 R in free floating rat brain slices using Dopamine ...read more
NAc D1 and D2 neuronal activation predicts performance in motivation-related tasks. Representative images of immunofluorescence for c-fos and dopamine receptor D1 (f) or c-fos and dopamine receptor D2 (i) in the NAc of ...read more
Hypoxia and HIF-1 alpha increase GH synthesis.a Effect of hypoxia (1% O2 for 18 h) on GH secretion on nine human acromegalic tumors in primary cell culture. For all cell culture experiments, each GH RIA value was ...read more

Product Details

Summary
Reactivity Mu, Rt, Bv(-), Ca(-), Hu(-), Po(-), Rb(-), Sh(-)Species Glossary
Applications WB, ICC/IF, IHC, IHC
Clone
SG2-D1a
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Dopamine D1R/DRD1 Antibody (SG2-D1a) Summary

Immunogen
Recombinant rat Dopamine Receptor D1 protein near the C-terminus. [Swiss-Prot# P18901]
Localization
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
Specificity
This antibody is specific for Dopamine Receptor D1A. There is no cross-reactivity with D1B (D5) receptor.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DRD1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 31462765)
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Western Blot 1:200-1:500
Application Notes
In Western blot multiple bands are seen around 45 and 100 kDa (dimer) in SF9 cell lysates overexpressing D1a and a single band at 90 kDa in striatal tissue lysate. Do NOT boil samples.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB110-60017 in the following applications:

Publications
Read Publications using
NB110-60017 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Dopamine D1R/DRD1 Antibody (SG2-D1a)

  • D(1A) dopamine receptor
  • D1DR
  • DADR
  • Dopamine D1 R
  • Dopamine D1 receptor
  • Dopamine D1R
  • dopamine receptor D1
  • DRD1
  • DRD1A

Background

Dopamine is a unique brain neurotransmitter which is responsible for the regulation of versatile processes such as motor activity, cognition, motivation, emotion, food intake, endocrine secretions etc. and dysfunctions in dopaminergic systems have been linked to drug addiction, schizophrenia, Parkinson's disease, Tourette's syndrome, attention-deficit and hyperactivity disorders as well as hyperprolactinemia. Dopamine Receptor D1 (DRD1), a GPCR protein, is the D1 subtype of dopamine receptors (DR) which represents the most abundant DR in CNS with capability of stimulating adenylyl cyclase and activating cyclic AMP-dependent protein kinases. DRD1 localize to cell membrane/ER membrane as multi-pass membrane protein and its transport from ER to cell surface is regulated by interaction with DNAJC14. DRD1 is highly expressed in the brain tissues, especially in the striatum, the nucleus accumbens, and the olfactory tubercle. DRD1 regulate neuronal growth and development, mediate behavioral responses, and modulate dopamine receptor D2-mediated events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dopamine D1R/DRD1 Antibody (NB110-60017)(16)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, KO, WB.


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ICC/IF
(5)
IF/IHC
(8)
IHC
(1)
IHC-Fr
(2)
KO
(1)
WB
(2)
All Applications
Filter By Species
Mouse
(6)
Rat
(9)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB110-60017 Applications Species
He ZX, Xi K, Liu KJ et al. A Nucleus Accumbens Tac1 Neural Circuit Regulates Avoidance Responses to Aversive Stimuli International journal of molecular sciences 2023-02-22 [PMID: 36901777] (IHC-Fr, Mouse) IHC-Fr Mouse
Struzyna LA, Browne KD, Burrell JC et al. Axonal Tract Reconstruction Using a Tissue-Engineered Nigrostriatal Pathway in a Rat Model of Parkinson's Disease International Journal of Molecular Sciences 2022-11-12 [PMID: 36430464] (IHC, ICC/IF) IHC, ICC/IF
Shen B, Zhang R, Yang G et al. Cannabidiol prevents methamphetamine-induced neurotoxicity by modulating dopamine receptor D1-mediated calcium-dependent phosphorylation of methyl-CpG-binding protein 2 Frontiers in Pharmacology 2022-09-06 [PMID: 36147353]
Smith CJ, Lintz T, Clark MJ et al. Prenatal opioid exposure inhibits microglial sculpting of the dopamine system selectively in adolescent male offspring Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 2022-07-14 [PMID: 35835992] (KO, IHC-Fr, Rat) KO, IHC-Fr Rat
Kasahara Y, Masukawa D, Kobayashi K et al. L-DOPA-induced Neurogenesis in the Hippocampus is Mediated through GPR143, a Distinct Mechanism of Dopamine Stem Cells 2022-01-19 [PMID: 35257172] (IF/IHC, Mouse) IF/IHC Mouse
Kisling A, Byrne S, Parekh RU Et al. Loss of Function in Dopamine D3 Receptor Attenuates Left Ventricular Cardiac Fibroblast Migration and Proliferation in vitro Frontiers in cardiovascular medicine 2021-10-11 [PMID: 34708087] (IF/IHC, ICC/IF, Mouse) IF/IHC, ICC/IF Mouse
Bourgeois JR, Kalyanasundaram G, Figueroa C et al. A semi-automated brain atlas-based analysis pipeline for c-Fos immunohistochemical data J Neurosci Methods 2020-10-20 [PMID: 33091429] (IF/IHC, Rat) IF/IHC Rat
Coimbra B, Soares-Cunha C, Vasconcelos NAP et al. Role of laterodorsal tegmentum projections to nucleus accumbens in reward-related behaviors Nat Commun 2019-09-12 [PMID: 31515512] (IF/IHC, Mouse, Rat) IF/IHC Mouse, Rat
Soares-Cunha C, de Vasconcelos NAP, Coimbra B et al. Nucleus accumbens medium spiny neurons subtypes signal both reward and aversion Mol. Psychiatry 2019-08-28 [PMID: 31462765] (ICC/IF, Mouse) ICC/IF Mouse
Soares-Cunha C, Coimbra B, Domingues A et al. Nucleus Accumbens Microcircuit Underlying D2-MSN-Driven Increase in Motivation. eNeuro 2018-04-19 [PMID: 29780881] (ICC/IF, Rat) ICC/IF Rat
Show All 16 Publications.

Reviews for Dopamine D1R/DRD1 Antibody (NB110-60017) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Rat, Other.

Reviews using NB110-60017:
Filter by Applications
IF
(1)
ICC
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Rat
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Dopamine D1R/DRD1 NB110-60017
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4
reviewed by:
Verified Customer
IF Rat 04/05/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedBrain free floating slices
SpeciesRat
LotA-2
Immunocytochemistry Dopamine D1R/DRD1 NB110-60017
Enlarge
5
reviewed by:
Verified Customer
ICC Other 04/10/2014
View

Summary

ApplicationImmunocytochemistry
Sample Testedbrain section
SpeciesOther
LotA-2
  5
reviewed by:
Verified Customer
IHC-Fr Rat 01/18/2010
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat retinal whole mounts and sections
SpeciesRat
LotA1

Product General Protocols

View specific protocols for Dopamine D1R/DRD1 Antibody (NB110-60017): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Dopamine D1R/DRD1 Antibody (NB110-60017). (Showing 1 - 1 of 1 FAQs).

  1. I am writing a manuscript and referring to results we obtained with your Dopamine Receptor D1 antibody. (These are results listed on your website: Journal of Neuroscience 29: 15001.) Your datasheet states that the immunogen was 123 residues of the rat Dopamine Receptor D1a. I realize the amino acid sequence that you used is proprietary, and do not need to know which residues you used. However, can you tell me the position of some of the amino acids so that I can state, with certainty, if these residues are not in the rat Dopamine Receptor D1b amino acid sequence?
    • I have spoken with our lab manager, and after pulling the original production data for this antibody, it does appear that the protein sequence used to synthesize the antibody was in fact the last 123 amino acids from residues 323 to 446 at the C-terminal. This updated immunogen information has been added to the datasheet to reflect that the antibody targets the C-terminus. Keep in mind the protein used contained to synthesize the antibody contained a HIS tag. I hope this information is helpful in your publication, and we are happy to hear that you are using our product for your published data.

Secondary Antibodies

 

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
04/05/2016
Application: IF
Species: Rat

Verified Customer
04/10/2014
Application: ICC
Species: Other

 
Verified Customer
01/18/2010
Application: IHC-Fr
Species: Rat

Bioinformatics

Gene Symbol DRD1