Dopamine D1R/DRD1 Antibody (SG2-D1a)

Images

 
Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of lysates from Sf9 cells which were transfected with rat DR-D1b (Lane 1) or DR-D1a (Lane 2) using DRD1 antibody (clone SG2-D1a). This clone ...read more
Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Representative images of immunofluorescence for c-fos and dopamine receptor D1 in the NAc. Image collected and cropped by CiteAb from the ...read more
Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine Receptor D1 in rat retinal whole mounts and sections. Image from ...read more
Immunohistochemistry Free-Floating: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine D1 R in free floating rat brain slices using Dopamine ...read more

Product Details

Summary
Reactivity Mu, Rt, Bv(-), Ca(-), Hu(-), Po(-), Rb(-), Sh(-)Species Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-FrFl
Clone
SG2-D1a
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Dopamine D1R/DRD1 Antibody (SG2-D1a) Summary

Immunogen
Recombinant rat Dopamine Receptor D1 protein near the C-terminus. [Swiss-Prot# P18901]
Localization
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
Specificity
This antibody is specific for Dopamine Receptor D1A. There is no cross-reactivity with D1B (D5) receptor.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DRD1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:500
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry Free-Floating
Application Notes
In Western blot multiple bands are seen around 45 and 100 kDa (dimer) in SF9 cell lysates overexpressing D1a and a single band at 90 kDa in striatal tissue lysate. Do NOT boil samples. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:31462765).
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB110-60017 in the following applications:

Publications
Read Publications using
NB110-60017 in the following applications:

  • 3 publications
  • IHC
    6 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Dopamine D1R/DRD1 Antibody (SG2-D1a)

  • D(1A) dopamine receptor
  • D1DR
  • DADR
  • Dopamine D1 R
  • Dopamine D1 receptor
  • Dopamine D1R
  • dopamine receptor D1
  • DRD1
  • DRD1A

Background

Dopamine is a unique brain neurotransmitter which is responsible for the regulation of versatile processes such as motor activity, cognition, motivation, emotion, food intake, endocrine secretions etc. and dysfunctions in dopaminergic systems have been linked to drug addiction, schizophrenia, Parkinson's disease, Tourette's syndrome, attention-deficit and hyperactivity disorders as well as hyperprolactinemia. Dopamine Receptor D1 (DRD1), a GPCR protein, is the D1 subtype of dopamine receptors (DR) which represents the most abundant DR in CNS with capability of stimulating adenylyl cyclase and activating cyclic AMP-dependent protein kinases. DRD1 localize to cell membrane/ER membrane as multi-pass membrane protein and its transport from ER to cell surface is regulated by interaction with DNAJC14. DRD1 is highly expressed in the brain tissues, especially in the striatum, the nucleus accumbens, and the olfactory tubercle. DRD1 regulate neuronal growth and development, mediate behavioral responses, and modulate dopamine receptor D2-mediated events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-60017
Species: Mu, Rt, Bv(-), Ca(-), Hu(-), Po(-), Rb(-), Sh(-)
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-FrFl

Publications for Dopamine D1R/DRD1 Antibody (NB110-60017)(10)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(3)
IHC
(6)
WB
(2)
All Applications
Filter By Species
Mouse
(3)
Rat
(8)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB110-60017 Applications Species
Bourgeois JR, Kalyanasundaram G, Figueroa C et al. A semi-automated brain atlas-based analysis pipeline for c-Fos immunohistochemical data J Neurosci Methods Oct 20 2020 [PMID: 33091429] (IHC, Rat) IHC Rat
Coimbra B, Soares-Cunha C, Vasconcelos NAP et al. Role of laterodorsal tegmentum projections to nucleus accumbens in reward-related behaviors Nat Commun Sep 12 2019 [PMID: 31515512] (IHC, Mouse, Rat) IHC Mouse, Rat
Soares-Cunha C, de Vasconcelos NAP, Coimbra B et al. Nucleus accumbens medium spiny neurons subtypes signal both reward and aversion Mol. Psychiatry Aug 28 2019 [PMID: 31462765] (ICC/IF, Mouse) ICC/IF Mouse
Soares-Cunha C, Coimbra B, Domingues A et al. Nucleus Accumbens Microcircuit Underlying D2-MSN-Driven Increase in Motivation. eNeuro Apr 19 2018 [PMID: 29780881] (ICC/IF, Rat) ICC/IF Rat
Morigaki R, Okita S, Goto S. Dopamine-Induced Changes in Galphaolf Protein Levels in Striatonigral and Striatopallidal Medium Spiny Neurons Underlie the Genesis of l-DOPA-Induced Dyskinesia in Parkinsonian Mice. Front Cell Neurosci. 2017 Feb 27 [PMID: 28239340] (IHC, Mouse) IHC Mouse
Feng XY, Zhang DN, Wang YA et al. Dopamine enhances duodenal epithelial permeability via the dopamine D5 receptor in rodent. Acta Physiol (Oxf) Oct 6 2016 [PMID: 27652590] (IHC, Rat) IHC Rat
Soares-Cunha C, Coimbra B, David-Pereira A et al. Activation of D2 dopamine receptor-expressing neurons in the nucleus accumbens increases motivation. Nat Commun Jun 24 2016 [PMID: 27337658] (ICC/IF, Rat) ICC/IF Rat
Stradleigh TW, Ishida AT. Fixation strategies for retinal immunohistochemistry Prog Retin Eye Res 2015 Apr 17 [PMID: 25892361] (IHC, Rat) IHC Rat
Hayashida Y, Rodriguez CV, Ogata G et al. Inhibition of adult rat retinal ganglion cells by D1-type dopamine receptor activation. J Neurosci;29(47):15001-16. 2009 Nov 25. [PMID: 19940196] (WB, Rat) WB Rat
Luedtke, RR et al. Immunoblot and immunohistochemical comparison of murine monoclonal antibodies specific for the rat D1a and D1b dopamine receptor subtypes. J Neuroimmunol;101(2):170-87. 1999 Nov 15. [PMID: 10580800] (WB, IHC, Rat) WB, IHC Rat

Reviews for Dopamine D1R/DRD1 Antibody (NB110-60017) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Rat, Other.

Reviews using NB110-60017:
Filter by Applications
IF
(1)
ICC
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Rat
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Dopamine D1R/DRD1 NB110-60017
Enlarge
4
reviewed by:
Anonymous
IF Rat 04/05/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedBrain free floating slices
SpeciesRat
LotA-2

Blocking

Blocking DetailsFree floating rat brain slices were blocked with 5% Fetal bovine serum (FBS), for 1hour at RT

Primary Anitbody

Dilution RatioAntibody was incubated at 4ºC overnight in PBS+0.3%Triton-X100+2%FBS (dilution 1:200)

Secondary Antibody

Secondary DescriptionGoat anti-Mouse IgG (H+L), Alexa Fluor® 594 conjugate
Secondary Manufacturer Cat#A-11005
Secondary Concentration1:500

Details

Detection NotesImage was acquired in confocal microscope; a negative control was used
Fixation DetailsFree floating rat brain slices were permeabilized with PBS+0.3%Triton-X100 for 30 min at RT
Wash DescriptionWash was made with PBS, 3 times, 10 minutes each time, at RT

Comments

CommentsThe immunofluorescence does not work if antigen retrieval is used.
Immunocytochemistry Dopamine D1R/DRD1 NB110-60017
Enlarge
5
reviewed by:
Anonymous
ICC Other 04/10/2014
View

Summary

ApplicationImmunocytochemistry
Sample Testedbrain section
SpeciesOther
LotA-2

Blocking

Blocking Details10% NGS in 0.3% TritonX-100 in 0.1M PBS, 1hr

Primary Anitbody

Dilution Ratio1:2K in 2% NGS in 0.3%TPBS 4C overnight

Secondary Antibody

Secondary Descriptiongoat anti-mouse
Secondary Manufacturer Cat#Vector
Secondary Concentration1:300

Details

Detection NotesABC, DAB staining
Fixation Detailsperfused by 4% PFA in 0.1M PBS, 0.3% H2O2 in 0.1M PBS 20min
Wash Description5min x 5 in either PBS or TPBS
Immunohistochemistry-Frozen Dopamine D1R/DRD1 NB110-60017
Enlarge
5
reviewed by:
Anonymous
IHC-Fr Rat 01/18/2010
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat retinal whole mounts and sections
SpeciesRat
LotA1

Blocking

Blocking DetailsBlocking Buffer: Normal Donkey Serum Blocking Time: 4 hours, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/500, Primary Ab Incubation Time: 18 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Product General Protocols

View specific protocols for Dopamine D1R/DRD1 Antibody (NB110-60017): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Dopamine D1R/DRD1 Antibody (NB110-60017). (Showing 1 - 1 of 1 FAQs).

  1. I am writing a manuscript and referring to results we obtained with your Dopamine Receptor D1 antibody. (These are results listed on your website: Journal of Neuroscience 29: 15001.) Your datasheet states that the immunogen was 123 residues of the rat Dopamine Receptor D1a. I realize the amino acid sequence that you used is proprietary, and do not need to know which residues you used. However, can you tell me the position of some of the amino acids so that I can state, with certainty, if these residues are not in the rat Dopamine Receptor D1b amino acid sequence?
    • I have spoken with our lab manager, and after pulling the original production data for this antibody, it does appear that the protein sequence used to synthesize the antibody was in fact the last 123 amino acids from residues 323 to 446 at the C-terminal. This updated immunogen information has been added to the datasheet to reflect that the antibody targets the C-terminus. Keep in mind the protein used contained to synthesize the antibody contained a HIS tag. I hope this information is helpful in your publication, and we are happy to hear that you are using our product for your published data.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-60017AF350
Alexa Fluor 405 NB110-60017AF405
Alexa Fluor 488 NB110-60017AF488
Alexa Fluor 532 NB110-60017AF532
Alexa Fluor 594 NB110-60017AF594
Alexa Fluor 647 NB110-60017AF647
Alexa Fluor 700 NB110-60017AF700
Alexa Fluor 750 NB110-60017AF750
Biotin NB110-60017B
DyLight 350 NB110-60017UV
DyLight 405 NB110-60017V
DyLight 488 NB110-60017G
DyLight 550 NB110-60017R
DyLight 594 NB110-60017DL594
DyLight 650 NB110-60017C
DyLight 680 NB110-60017FR
DyLight 755 NB110-60017IR
FITC NB110-60017F
HRP NB110-60017H
Janelia Fluor 549 NB110-60017JF549
Janelia Fluor 646 NB110-60017JF646

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
04/05/2016
Application: IF
Species: Rat

Anonymous
04/10/2014
Application: ICC
Species: Other

Anonymous
01/18/2010
Application: IHC-Fr
Species: Rat

Bioinformatics

Gene Symbol DRD1