Dopamine D1R/DRD1 Antibody (JM10-93)

Western Blot: Dopamine D1R/DRD1 Antibody (JM10-93) [NBP2-66807] - Analysis of DRD1 on mouse kidney cells lysates using anti-DRD1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Dopamine D1R/DRD1 Antibody (JM10-93) [NBP2-66807] - Staining DRD1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Dopamine D1R/DRD1 Antibody (JM10-93) [NBP2-66807] - Analysis of paraffin-embedded rat brain tissue using anti-DRD1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Dopamine D1R/DRD1 Antibody (JM10-93) [NBP2-66807] - Staining DRD1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Dopamine D1R/DRD1 Antibody (JM10-93) [NBP2-66807] - Staining DRD1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Dopamine D1R/DRD1 Antibody (JM10-93) [NBP2-66807] - Analysis of paraffin-embedded mouse brain tissue using anti-DRD1 antibody. Counter stained with hematoxylin.

Product Discontinued

Dopamine D1R/DRD1 Antibody (JM10-93) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within C-terminal human Dopamine D1R/DRD1. (SwissProt: P21728 Human; SwissProt: Q61616 Mouse; SwissProt: P18901 Rat)
• Localization: Cell membrane. Endoplasmic reticulum membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DRD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Dopamine D1R/DRD1 Antibody (JM10-93)

• D(1A) dopamine receptor
• D1DR
• DADR
• Dopamine D1 R
• Dopamine D1 receptor
• Dopamine D1R
• dopamine receptor D1
• DRD1
• DRD1A

Background

The members of the G protein-coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin, and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. Beta-adrenergic receptor bound to adrenaline activates adenylyl cyclase, while alpha 2-adrenergic receptor bound to adrenaline inhibits adenylyl cyclase. The dopamine receptors are divided into two classes; D1 and D2, which differ in their functional characteristics. D1 receptors stimulate adenylyl cyclase, while D2 receptors inhibit adenylyl cyclase activity. Five different subtypes of dopamine receptor have been described to date. D1DR and D5DR belong to the D1 subclass, while D2DR, D3DR and D4DR belong to the D2 subclass of dopamine receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DRD1