Dopamine D1R/DRD1 Antibody (887438) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human Dopamine D1/DRD1 and eGFP was stained with either (A) Mouse Anti-Human Dopamine D1 R/DRD1 Monoclonal Antibody (Catalog # MAB8276) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Dopamine D1 R/DRD1 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human Dopamine D1 R/DRD1 Monoclonal Antibody (Catalog # MAB8276) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Expression of MCT8 and OATP1C1 in D1-MSN (direct pathway medium-sized spiny neurons) in human and macaque striatum. Representative confocal microscope compositions from double-stained sections for MCT8 (green) or OATP1C1 (green) and for the D1-MSN marker DRD1 (red) in caudate nucleus or putamen. Merged images (right side) show the colocalization of both signals. Coexpression of MCT8 and DRD1 is observed in human (A–C) and macaque (D–F) striatum. Coexpression of OATP1C1 and DRD1 is observed in human (G–I) and macaque (J–L) striatum. Counterstaining with DAPI (blue) shows nuclei of all cells. Note that in humans the MCT8 signal is located mainly at the cell membrane, while in macaques it is located at the membrane and in the cytoplasm. Cd: caudate nucleus; DRD1: Dopamine receptor type 1; D1-MSN: D1 receptor-expressing medium-sized spiny neurons; Put: putamen. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37298594), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 887438
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Dopamine D1R/DRD1 Antibody (887438) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Dopamine D1 R/DRD1
  Met1-Thr446
  Accession # P21728
• Specificity: Stains human Dopamine D1 R/DRD1 transfected cells but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DRD1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dopamine D1R/DRD1 Antibody (887438) [Unconjugated]

• D(1A) dopamine receptor
• D1DR
• DADR
• Dopamine D1 R
• Dopamine D1 receptor
• Dopamine D1R
• dopamine receptor D1
• DRD1
• DRD1A

Background

Dopamine Receptor D1 (DRD1) is a 50 kDa member of class 1 GPCR superfamily and is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. DRD1 is associated with nicotine dependence, schizophrenia, and systolic blood pressure levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dopamine D1R/DRD1 Antibody (MAB8276)(3)

Publications using MAB8276

• Wang, T;Wang, Y;Montero-Pedrazuela, A;Prensa, L;Guadaño-Ferraz, A;Rausell, E; Thyroid Hormone Transporters MCT8 and OATP1C1 Are Expressed in Projection Neurons and Interneurons of Basal Ganglia and Motor Thalamus in the Adult Human and Macaque Brains International journal of molecular sciences 2023-06-01 [PMID: 37298594] (IHC, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Saimiri sciureus (South American Squirrel Monkey), Human, Primate - Macaca mulatta (Rhesus Macaque))
• Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al. Development and application of a high-content virion display human GPCR array Nature Communications 4/30/2019 [PMID: 31040288]
• Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al. Development and application of a high-content virion display human GPCR array Nature Communications 2019-04-30 [PMID: 31040288] (Assay Development, Human)

Bioinformatics

• Gene Symbol: DRD1
• Uniprot:
  • P21728()