DOK7 Antibody (OTI1A9) - Azide and BSA Free

Images

 
Western Blot: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK7 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - Staining of paraffin-embedded Human pancreas tissue using anti-DOK7 mouse monoclonal antibody.
Flow Cytometry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK7.
Western Blot: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-DOK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: ...read more
Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DOK7 mouse monoclonal antibody.
Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DOK7 mouse monoclonal antibody.
Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - Staining of paraffin-embedded Human liver tissue using anti-DOK7 mouse monoclonal antibody.
Immunohistochemistry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - Staining of paraffin-embedded Human prostate tissue using anti-DOK7 mouse monoclonal antibody.
Flow Cytometry: DOK7 Antibody (OTI1A9) - Azide and BSA Free [NBP2-72494] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DOK7 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

DOK7 Antibody (OTI1A9) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human DOK7(NP_775931) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DOK7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • CyTOF-ready
Theoretical MW
52.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for DOK7 Antibody (OTI1A9) - Azide and BSA Free

  • C4orf25
  • C4orf25chromosome 4 open reading frame 25
  • CMS1B
  • docking protein 7
  • DOK7
  • Dok-7
  • Downstream of tyrosine kinase 7
  • FLJ33718
  • FLJ39137
  • FLJ90556
  • protein Dok-7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DOK7 Antibody (NBP2-72494) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-72494AF350
Alexa Fluor 405 NBP2-72494AF405
Alexa Fluor 488 NBP2-72494AF488
Alexa Fluor 532 NBP2-72494AF532
Alexa Fluor 594 NBP2-72494AF594
Alexa Fluor 647 NBP2-72494AF647
Alexa Fluor 700 NBP2-72494AF700
Alexa Fluor 750 NBP2-72494AF750
Allophycocyanin NBP2-72494APC
Allophycocyanin/Cy7 NBP2-72494APCCY7
Biotin NBP2-72494B
DyLight 350 NBP2-72494UV
DyLight 405 NBP2-72494V
DyLight 488 NBP2-72494G
DyLight 550 NBP2-72494R
DyLight 594 NBP2-72494DL594
DyLight 650 NBP2-72494C
DyLight 680 NBP2-72494FR
DyLight 755 NBP2-72494IR
FITC NBP2-72494F
HRP NBP2-72494H
Janelia Fluor 549 NBP2-72494JF549
Janelia Fluor 646 NBP2-72494JF646
PE NBP2-72494PE
PE/Atto594 NBP2-72494PEATT594
PE/Cy5.5 NBP2-72494PECY55
PE/Cy7 NBP2-72494PECY7
PerCP NBP2-72494PCP

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Bioinformatics

Gene Symbol DOK7