DOK7 Antibody (OTI1A9)

Western Blot: DOK7 Antibody (1A9) [NBP2-02073] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-DOK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: DOK7 Antibody (1A9) [NBP2-02073] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK7.

Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Human prostate tissue using anti-DOK7 mouse monoclonal antibody.

Flow Cytometry: DOK7 Antibody (1A9) [NBP2-02073] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DOK7 antibody, and then analyzed by flow cytometry.

Western Blot: DOK7 Antibody (1A9) [NBP2-02073] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK7.

Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DOK7 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DOK7 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Human liver tissue using anti-DOK7 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Human pancreas tissue using anti-DOK7 mouse monoclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

DOK7 Antibody (OTI1A9) Summary

• Immunogen: Full length human recombinant protein of human DOK7(NP_775931) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DOK7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 52.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DOK7 Antibody (OTI1A9)

• C4orf25
• C4orf25chromosome 4 open reading frame 25
• CMS1B
• docking protein 7
• DOK7
• Dok-7
• Downstream of tyrosine kinase 7
• FLJ33718
• FLJ39137
• FLJ90556
• protein Dok-7

Background

The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DOK7 Antibody (NBP2-02073)(2)

Publications using NBP2-02073

• Juhlen R, Breckpot J, Fahrenkrog B Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts bioRxiv (ICC/IF)
• Juhlen R, Breckpot J, Fahrenkrog B Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts Sci Rep 2020-11-10 [PMID: 33168876] (WB, Human)

Bioinformatics

• Gene Symbol: DOK7
• Entrez:
  • 285489(Human)