DOK7 Antibodies

HAF109). A specific band was detected for DOK7 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox"> HAF109). A specific band was detected for DOK7 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of untreated MCF-7 human breast cancer cell line and T47D human breast cancer cell line and C2C12 mouse myoblast cell line and L6 rat myoblast cell line untreated (-) or treated (+) with 2% horse serum for 6 days. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat DOK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DOK7 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="DOK7 was detected in immersion fixed C2C12 mouse myoblast cell line differentiated into myotubes using Mouse Anti-Human DOK7 Monoclonal Antibody (Catalog # MAB6398) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />