DOK1 Antibody

Images

 
Western Blot: DOK1 Antibody [NBP1-77064] - Analysis of DOK1 in A) Daudi and B) NIH 3T3 using NBP1-77064 at 0.5 ug/ml.
Immunocytochemistry/ Immunofluorescence: DOK1 Antibody [NBP1-77064] - Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.
Western Blot: DOK1 Antibody [NBP1-77064] - Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: DOK1 Antibody [NBP1-77064] - K562 cells with DOK1 antibody at 2 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DOK1 Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DOK1. The immunogen is located within amino acids 410 - 460 of DOK1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DOK1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
DOK1 antibody can be used for detection of DOK1 expression by Western blot at 0.5 - 1 mg/ml. A 62 kDa band should be detected.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
DOK1 Peptide (NBP1-77064PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DOK1 Antibody

  • docking protein 1 (downstream of tyrosine kinase 1)
  • docking protein 1
  • docking protein 1, 62kD (downstream of tyrosine kinase 1)
  • docking protein 1, 62kDa (downstream of tyrosine kinase 1)
  • Downstream of tyrosine kinase 1
  • MGC117395
  • MGC138860
  • p62(dok)
  • P62DOK
  • pp62

Background

Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DOK1
Uniprot