DNMT3B Antibody

Images

 
Western Blot: DNMT3B Antibody [NB100-266] - WB analysis of 40ug nuclear lysates of HCT116 cells which were left untransfected (lane 1) or were transiently transfected to overexpress DNMT3B2 and DNMT3B3, (lane 2 and ...read more
Immunocytochemistry/ Immunofluorescence: Dnmt3b Antibody [NB100-266] - Dnmt3b antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: Dnmt3b Antibody [NB100-266] - Dnmt3b antibody was tested in mouse testis using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DNMT3B Antibody Summary

Immunogen
Amino acids 4-101 of human Dnmt3b [UniProt# Q9UBC3]
Localization
Nuclear, cytoplasmic.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNMT3B
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:1000
Application Notes
This Dnmt3b antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot, where a band is seen at ~100 kDa. This antibody does not neutralize the enzymatic activity of its corresponding methyltransferase. In ICC/IF, cytoplasmic staining was observed in NIH-3T3 cells. In IHC, predominant nuclear staining was observed, as well as some weaker cytoplasmic signal, in mouse testes. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-266 in the following applications:

Publications
Read Publications using
NB100-266 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for DNMT3B Antibody

  • DNA (cytosine-5-)-methyltransferase 3 beta
  • DNA (cytosine-5)-methyltransferase 3B
  • DNA methyltransferase HsaIIIB
  • DNA MTase HsaIIIB
  • DNMT3B
  • EC 2.1.1.37
  • ICF
  • ICF1
  • M.HsaIIIB

Background

Owing to its critical role in long-term transcriptional silencing, DNA methylation represents a key layer of epigenetic information that is essential to biological processes such as embryonic development, germline differentiation, functional maturation of neuronal tissues etc. and DNA-methyltransferases family members (DNMT3A, DNMT3B and DNMT3L) have the ability to transfer methyl group derived from folate intermediates to nucleic acids. Among DNMTs, Dnmt3b [DNA (cytosine-5)-methyltransferase 3B] primarily functions in de novo DNA methylation and its expression is developmentally regulated. Dnmt3b is essential for establishment of DNA methylation patterns during development and may preferentially methylates nucleosomal DNA within the nucleosome core region. Independent of DNA methylation, Dnmt3b functions as transcriptional co-repressor by associating with CBX4, ZHX1 etc. In association with DNMT1 and via the recruitment of CTCFL/BORIS, Dnmt3b is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Mutations in DNMT3B have been linked to the immunodeficiency-centromeric instability-facial anomalies syndrome type 1 (ICF1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNMT3B Antibody (NB100-266)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ChIP, ICC/IF, IHC-P, WB.


Filter By Application
ChIP
(1)
ICC/IF
(2)
IHC-P
(1)
WB
(9)
All Applications
Filter By Species
Human
(7)
Mouse
(7)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-266 Applications Species
Liu Y, Sun L, Fong P et al. An association between overexpression of DNA methyltransferase 3B4 and clear cell renal cell carcinoma. Oncotarget. Mar 21 2017 [PMID: 28160561] (WB, Human) WB Human
Hoang TV, Horowitz ER, Chaffee BR et al. Lens development requires DNMT1 but takes place normally in the absence of both DNMT3A and DNMT3B activity. Epigenetics. Nov 8 2016 [PMID: 27824296] (Mouse) Mouse
Rinaldi L, Datta D, Serrat J et al. Dnmt3a and Dnmt3b Associate with Enhancers to Regulate Human Epidermal Stem Cell Homeostasis. CellPress 2016 Jul 28 [PMID: 27476967] (ICC/IF, ChIP, WB, Human) ICC/IF, ChIP, WB Human
Quist T, Jin H, Zhu Jf et al. The impact of osteoblastic differentiation on osteosarcomagenesis in the mouse. Oncogene. 2014 Oct 27 [PMID: 25347737] (IHC-P, Mouse) IHC-P Mouse
Chen M, Shabashvili D, Nawab A, Yang SX, Dyer LM, Brown KD, Hollingshead M, Hunter KW, Kaye FJ, Hochwald SN, Marquez VE, Steeg P, Zajac-Kaye M. DNA methyltransferase inhibitor, zebularine, delays tumor growth and induces apoptosis in a genetically engineered mouse model of breast cancer. Mol Cancer Ther;11(2):370-82. 2012 Feb. [PMID: 22203734]
Kaminskyy V, Surova O, Vaculova A, Zhivotovsky B. Combined inhibition of DNA methyltransferase and histone deacetylase restores caspase-8 expression and sensitizes SCLC cells to TRAIL. Carcinogenesis. 2011 Jul 18. [PMID: 21771726]
Al-Salihi M, Yu M, Burnett DM et al. The depletion of DNA methyltransferase-1 and the epigenetic effects of 5-aza-2'deoxycytidine (decitabine) are differentially regulated by cell cycle progression. Epigenetics 2011 Aug [PMID: 21725200] (Human) Human
Kinney SM, Chin HG, Vaisvila R et al. Tissue-specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genomes. J Biol Chem. Jul. 2011 [PMID: 21610077] (WB, Mouse) WB Mouse
Kim DH, Jung YJ, Lee JE et al. SIRT1 activation by resveratrol ameliorates cisplatin-induced renal injury through deacetylation of p53. Am J Physiol Renal Physiol;301(2):F427-35. 2011 Aug. [PMID: 21593185]
Morey Kinney SR, Smiraglia DJ, James SR et al. Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypomethylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate model. Mol Cancer Res;6(8):1365-74. 2008 Aug. [PMID: 18667590] (WB, Mouse) WB Mouse
Show All 16 Publications.

Review for DNMT3B Antibody (NB100-266) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-266:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 11/30/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsGood!

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in 0.1% PBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL Plus

Comments

CommentsGood!

Product General Protocols

View specific protocols for DNMT3B Antibody (NB100-266): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNMT3B Antibody (NB100-266). (Showing 1 - 1 of 1 FAQs).

  1. I'd like to know if this antibody (catalog # NBP1-00783) is likely to cross-react with DNMT3a? Also, I'd like to use it to detect marsupial DNMT3b (XP_003757074.1) by WB and IHC-P. Is it likely to cross-react with this? Or with marsupial DNMT3a (XP_003757846)?
    • Our best antibody to Dnmt3b is NB100-266 but unfortunately, neither NB100-266 nor NBP1-00783 are predicted to cross-react with your species. NBP1-54350 is predicted to cross-react react with marsupial, but the immunogen also shares 86% identity with human and marsupial Dnmt3a. NBP1-54350 would have to be the antibody I most recommend.

Secondary Antibodies

 

Isotype Controls

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Blogs on DNMT3B.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b
Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r...  Read full blog post.

DNMT3B: Roles in Leukemia
DNA-methyltransferase 3B (DNMT3B), also known as DNA methyltransferase HsaIIIB, is a member of the class I-like SAM-binding methyltransferase superfamily and C5-methyltransferase family. DNMT3B plays an essential role in the establishment of DNA methy...  Read full blog post.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

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Recent Reviews

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Anonymous
11/30/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol DNMT3B
Entrez
Uniprot