DNMT3B Antibody


Western Blot: Dnmt3b Antibody [NB100-266] - Analysis using NB100-266 to detect overexpressed DNMT-38 in HCT116 cells. Lane 1: 40 ug of nuclear extract from untransfected T24 degrees Cells. Lane 2-3: 40 ug of nuclear ...read more
Immunocytochemistry/ Immunofluorescence: Dnmt3b Antibody [NB100-266] - Dnmt3b antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: Dnmt3b Antibody [NB100-266] - Dnmt3b antibody was tested in mouse testis using DAB with hematoxylin counterstain.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

DNMT3B Antibody Summary

Amino acids 4-101 of human Dnmt3b [UniProt# Q9UBC3]
Nuclear, cytoplasmic.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No Preservative
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
This Dnmt3b antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot, where a band is seen at ~100 kDa. This antibody does not neutralize the enzymatic activity of its corresponding methyltransferase. In ICC/IF, cytoplasmic staining was observed in NIH-3T3 cells. In IHC, predominant nuclear staining was observed, as well as some weaker cytoplasmic signal, in mouse testes. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
100 kDa
Reviewed Applications
Read 1 Review rated 5
NB100-266 in the following application:

Read Publications using
NB100-266 in the following applications:

Reactivity Notes

Human and mouse.

Alternate Names for DNMT3B Antibody

  • DNA (cytosine-5-)-methyltransferase 3 beta
  • DNA (cytosine-5)-methyltransferase 3B
  • DNA methyltransferase HsaIIIB
  • DNA MTase HsaIIIB
  • Dnmt3b
  • EC
  • ICF
  • ICF1
  • M.HsaIIIB


Owing to its critical role in long-term transcriptional silencing, DNA methylation represents a key layer of epigenetic information that is essential to biological processes such as embryonic development, germline differentiation, functional maturation of neuronal tissues etc. and DNA-methyltransferases family members (DNMT3A, DNMT3B and DNMT3L) have the ability to transfer methyl group derived from folate intermediates to nucleic acids. Among DNMTs, Dnmt3b [DNA (cytosine-5)-methyltransferase 3B] primarily functions in de novo DNA methylation and its expression is developmentally regulated. Dnmt3b is essential for establishment of DNA methylation patterns during development and may preferentially methylates nucleosomal DNA within the nucleosome core region. Independent of DNA methylation, Dnmt3b functions as transcriptional co-repressor by associating with CBX4, ZHX1 etc. In association with DNMT1 and via the recruitment of CTCFL/BORIS, Dnmt3b is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Mutations in DNMT3B have been linked to the immunodeficiency-centromeric instability-facial anomalies syndrome type 1 (ICF1).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
Species: Hu, Mu
Applications: WB, ChIP, Flow, ICC/IF, IHC-P, IHC-WhMt
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, IHC, IP
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Pm
Applications: WB, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Xp, Ye
Applications: WB, ChIP, ICC/IF, IHC, IHC-P

Publications for DNMT3B Antibody (NB100-266)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-266 Applications Species
Quist T, Jin H, Zhu Jf et al. The impact of osteoblastic differentiation on osteosarcomagenesis in the mouse. Oncogene. 2014 Oct 27 [PMID:25347737] (IHC-P, Mouse) IHC-P Mouse
Chen M, Shabashvili D, Nawab A, Yang SX, Dyer LM, Brown KD, Hollingshead M, Hunter KW, Kaye FJ, Hochwald SN, Marquez VE, Steeg P, Zajac-Kaye M. DNA methyltransferase inhibitor, zebularine, delays tumor growth and induces apoptosis in a genetically engineered mouse model of breast cancer. Mol Cancer Ther;11(2):370-82. 2012 Feb. [PMID:22203734]
Kaminskyy V, Surova O, Vaculova A, Zhivotovsky B. Combined inhibition of DNA methyltransferase and histone deacetylase restores caspase-8 expression and sensitizes SCLC cells to TRAIL. Carcinogenesis. 2011 Jul 18. [PMID:21771726]
Al-Salihi M, Yu M, Burnett DM et al. The depletion of DNA methyltransferase-1 and the epigenetic effects of 5-aza-2'deoxycytidine (decitabine) are differentially regulated by cell cycle progression. Epigenetics 2011 Aug [PMID:21725200] (Human) Human
Kinney SM, Chin HG, Vaisvila R et al. Tissue-specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genomes. J Biol Chem. Jul. 2011 [PMID:21610077] (WB, Mouse) WB Mouse
Kim DH, Jung YJ, Lee JE et al. SIRT1 activation by resveratrol ameliorates cisplatin-induced renal injury through deacetylation of p53. Am J Physiol Renal Physiol;301(2):F427-35. 2011 Aug. [PMID:21593185]
Morey Kinney SR, Smiraglia DJ, James SR et al. Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypomethylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate model. Mol Cancer Res;6(8):1365-74. 2008 Aug. [PMID:18667590] (WB, Mouse) WB Mouse
Morey SR, Smiraglia DJ, James SR et al. DNA methylation pathway alterations in an autochthonous murine model of prostate cancer. Cancer Res;66(24):11659-67. 2006 Dec 15. [PMID:17178860] (WB, Mouse) WB Mouse
Xiong Y, Dowdy SC, Podratz KC et al. Histone deacetylase inhibitors decrease DNA methyltransferase-3B messenger RNA stability and down-regulate de novo DNA methyltransferase activity in human endometrial cells. Cancer Res;65(7):2684-9. 2005 Apr 1. [PMID:15805266] (WB, Human) WB Human
Qiu YY, Mirkin BL, Dwivedi RS et al. Differential expression of DNA-methyltransferases in drug resistant murine neuroblastoma cells. Cancer Detect Prev;26(6):444-53. 2002 [PMID:12507229] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Show All 13 Publications.

Reviews for DNMT3B Antibody (NB100-266) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-266:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
reviewed by:
WB Human 11/30/2011


ApplicationWestern Blot
Sample TestedHuman


Blocking DetailsBlocking Buffer: 5% BSA in 0.1% PBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:5000


Detection NotesDetection Method: ECL Plus



Product General Protocols

View specific protocols for DNMT3B Antibody (NB100-266): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNMT3B Antibody (NB100-266). (Showing 1 - 1 of 1 FAQs).

  1. I'd like to know if this antibody (catalog # NBP1-00783) is likely to cross-react with DNMT3a? Also, I'd like to use it to detect marsupial DNMT3b (XP_003757074.1) by WB and IHC-P. Is it likely to cross-react with this? Or with marsupial DNMT3a (XP_003757846)?
    • Our best antibody to Dnmt3b is NB100-266 but unfortunately, neither NB100-266 nor NBP1-00783 are predicted to cross-react with your species. NBP1-54350 is predicted to cross-react react with marsupial, but the immunogen also shares 86% identity with human and marsupial Dnmt3a. NBP1-54350 would have to be the antibody I most recommend.

Secondary Antibodies


Isotype Controls

Additional DNMT3B Antibody Products

Related Products by Gene

Bioinformatics Tool for DNMT3B Antibody (NB100-266)

Discover related pathways, diseases and genes to DNMT3B Antibody (NB100-266). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DNMT3B Antibody (NB100-266)

Discover more about diseases related to DNMT3B Antibody (NB100-266).

Pathways for DNMT3B Antibody (NB100-266)

View related products by pathway.

PTMs for DNMT3B Antibody (NB100-266)

Learn more about PTMs related to DNMT3B Antibody (NB100-266).

Research Areas for DNMT3B Antibody (NB100-266)

Find related products by research area.

Blogs on DNMT3B.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b
Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r...  Read full blog post.

DNMT3B: Roles in Leukemia
DNA-methyltransferase 3B (DNMT3B), also known as DNA methyltransferase HsaIIIB, is a member of the class I-like SAM-binding methyltransferase superfamily and C5-methyltransferase family. DNMT3B plays an essential role in the establishment of DNA methy...  Read full blog post.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol DNMT3B

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-266 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought