Dnmt2 Antibody - Azide and BSA Free

Western Blot: Dnmt2 Antibody [NBP2-92029] - Analysis of extracts of various cell lines, using Dnmt2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.

Product Discontinued

Dnmt2 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 192-391 of human TRDMT1 (NP_004403.1). VHPQKYAMDVENKIQEKNVEPNISFDGSIQCSGKDAILFKLETAEEIHRKNQQDSDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYALLLDIVQPTCRRSVCFTKGYGSYIEGTGSVLQTAEDVQVENIYKSLTNLSQEEQITKLLILKLRYFTPKEIANLLGFPPEFGFPEKITVKQRYRLLGNSLNVHVVAKLIKILYE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRDMT1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Dnmt2 Antibody - Azide and BSA Free

• DMNT2
• DNA (cytosine-5-)-methyltransferase 2
• DNA (cytosine-5)-methyltransferase-like protein 2
• DNA methyltransferase homolog HsaIIP
• DNA methyltransferase-2
• DNA MTase homolog HsaIIP
• DNMT2
• EC 2.1.1
• EC 2.1.1.29
• M.HsaIIP
• MHSAIIP
• PUMET
• RNMT1
• tRNA (cytosine-5-)-methyltransferase
• tRNA aspartic acid methyltransferase 1

Background

tRNA aspartic acid methyltransferase 1 (TRDMT1), also known as DNMT2, is a DNA methyltransferase homolog that does not methylate DNA. Instead DNMT-2 methylates cytosine-38 in the anticodon loop of aspartic acid transfer RNA. Spec ifically, DNMT-2 methylates cytosine 38 in the anticodon loop of tRNA(Asp)

DNMT2 antibodies are useful for RNA research, cell replication studies, and research on certian cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: TRDMT1