DNAJC3 Antibody - BSA Free

Western Blot: DNAJC3 Antibody [NBP1-33393] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Molt-4 E: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Po, Bv, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DNAJC3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DNAJC3 Antibody - BSA Free (NBP1-33393) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DNAJC3. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum
• Marker: ER Stress Marker
• Predicted Species: Mouse (98%), Rat (94%), Porcine (98%), Bovine (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNAJC3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (82%), Xenopus laevis (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DNAJC3 Antibody - BSA Free

• DnaJ (Hsp40) homolog, subfamily C, member 3
• HP58
• Interferon-induced, double-stranded RNA-activated protein kinase inhibitor
• P58FLJ21288
• P58IPKProtein kinase inhibitor p58
• PRKRIdnaJ homolog subfamily C member 3
• Protein kinase inhibitor of 58 kDa
• protein-kinase, interferon-inducible double stranded RNA dependent inhibitor

Background

The 58-kDa inhibitor of the interferon-induced double-stranded RNA-activated protein kinase (PKR) is a cellular protein that is activated during influenza virus infection to down-regulate the activity of PKR. The 58-kDa inhibitor of the interferon-induced double-stranded RNA-activated protein kinase (PKR) is a cellular protein that is activated during influenza virus infection to down-regulate the activity of PKR (1). The P58 protein inhibits both the autophosphorylation of PKR and the phosphorylation of the PKR natural substrate, the alpha subunit of eukaryotic initiation factor eIF-2. Sequence analysis revealed that P58 is a member of the tetratricopeptide family of proteins (2). Also, like other J-domain proteins, P58 stimulated the ATPase activity of Hsc70. Taken together, data suggests that P58 is a co-chaperone, possibly directing hsp/Hsc70 to refold, and thus inhibit kinase function (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DNAJC3
• Entrez:
  • 5611(Human)
• Uniprot:
  • Q13217(Human)