DNAJB11 Antibody - BSA Free

Western Blot: DNAJB11 Antibody [NBP1-84899] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/Immunofluorescence: DNAJB11 Antibody [NBP1-84899] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: DNAJB11 Antibody [NBP1-84899] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Western Blot: DNAJB11 Antibody [NBP1-84899] - Analysis in human cell line HeLa.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DNAJB11 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DNAJB11 Antibody - BSA Free (NBP1-84899) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-DNAJB11 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKVYNGLQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNAJB11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  DNAJB11 Protein (NBP1-84899PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for DNAJB11 Antibody - BSA Free

• ABBP-2
• APOBEC1-binding protein 2
• DnaJ (Hsp40) homolog, subfamily B, member 11
• dnaJ homolog subfamily B member 11
• DnaJ protein homolog 9
• EDJABBP2
• ER-associated dnaJ protein 3
• ER-associated DNAJ
• ER-associated Hsp40 co-chaperone
• ERdj3DJ9
• ERJ3
• ERj3p
• hDj9
• hDj-9
• HEDJERj3
• Human DnaJ protein 9
• PRO1080
• PWP1-interacting protein 4
• UNQ537

Background

Members of the heat shock protein 40 (HSP 40) family of proteins all contain a highly conserved J domain that associates with HSP 70 and regulates the function of HSP 70 by activating its adenosine triphosphatase activity. ERdj3, an HSP 40 chaperone, is expressed in the ER lumen, where it interacts with BiP, a molecule involved in retrotranslocating proteins out of the ER. ERdj3 also associates with several other protein substrates, including unfolded light chains, a nonsecreted Ig light chain mutant and a VSV-G ts045 mutant. Shiga toxin (Stx) is a bacterial tool that enzymatically inactivates the 28S rRNA, inhibiting protein synthesis of infected cells. Stx also interacts with ERdj3 and Sec 61 to form a complex through which proteins are retrotranslocated to the cytoplasm. ERdj3 may play a role in the ER quality control system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNAJB11 Antibody (NBP1-84899)(1)

Publication using NBP1-84899

• Wen KW, Damania B et al. Hsp90 and Hsp40/Erdj3 are required for the expression and anti-apoptotic function of KSHV K1. Oncogene 2010-06-01 [PMID: 20418907]

Bioinformatics

• Gene Symbol: DNAJB11