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DNAJB11 Antibody

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Western Blot: DNAJB11 Antibody [NBP1-84899] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Western Blot: DNAJB11 Antibody [NBP1-84899] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: DNAJB11 Antibody [NBP1-84899] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DNAJB11 Antibody [NBP1-84899] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: DNAJB11 Antibody [NBP1-84899] - Analysis in human cell line HeLa.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DNAJB11 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKVYNGLQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNAJB11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DNAJB11 Protein (NBP1-84899PEP)
Publications
Read Publication using NBP1-84899.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DNAJB11 Antibody

  • ABBP-2
  • APOBEC1-binding protein 2
  • DnaJ (Hsp40) homolog, subfamily B, member 11
  • dnaJ homolog subfamily B member 11
  • DnaJ protein homolog 9
  • EDJABBP2
  • ER-associated dnaJ protein 3
  • ER-associated DNAJ
  • ER-associated Hsp40 co-chaperone
  • ERdj3DJ9
  • ERJ3
  • ERj3p
  • hDj9
  • hDj-9
  • HEDJERj3
  • Human DnaJ protein 9
  • PRO1080
  • PWP1-interacting protein 4
  • UNQ537

Background

Members of the heat shock protein 40 (HSP 40) family of proteins all contain a highly conserved J domain that associates with HSP 70 and regulates the function of HSP 70 by activating its adenosine triphosphatase activity. ERdj3, an HSP 40 chaperone, is expressed in the ER lumen, where it interacts with BiP, a molecule involved in retrotranslocating proteins out of the ER. ERdj3 also associates with several other protein substrates, including unfolded light chains, a nonsecreted Ig light chain mutant and a VSV-G ts045 mutant. Shiga toxin (Stx) is a bacterial tool that enzymatically inactivates the 28S rRNA, inhibiting protein synthesis of infected cells. Stx also interacts with ERdj3 and Sec 61 to form a complex through which proteins are retrotranslocated to the cytoplasm. ERdj3 may play a role in the ER quality control system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on HeLa with NBP1-06274 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.
Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - NIH-3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-GRP78/HSPA5 at 2 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Flow Cytometry: Hsp40/DNAJB1 Antibody (1F9) [NBP2-03433] - Hsp40/DNAJB1 Antibody (1F9) HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Hsp40 antibody, and then analyzed by flow cytometry.
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Immunohistochemistry-Paraffin: Hsp40/DNAJB1 Antibody (1F9) [NBP2-03433] - Hsp40/DNAJB1 Antibody (1F9) Staining of paraffin-embedded Human prostate tissue using anti-Hsp40 mouse monoclonal antibody.
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Flow Cytometry: DNAJA2 Antibody (3A10) [NBP2-02996] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DNAJA2 antibody, and then analyzed by flow cytometry.
Western Blot: DNAJA2 Antibody (3A10) [NBP2-02996] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJA2.
Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DNAJA2 mouse monoclonal antibody.
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
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H00010049-M01
ELISA: DNAJB6 Antibody (2C11-C1) [H00010049-M01] - Detection limit for recombinant GST tagged DNAJB6 is approximately 1ng/ml as a capture antibody.
Western Blot: DNAJB6 Antibody (2C11-C1) [H00010049-M01] - DNAJB6 monoclonal antibody (M01), clone 2C11-C1. Analysis of DNAJB6 expression in C32.
Western Blot: DNAJB6 Antibody (2C11-C1) [H00010049-M01] - Analysis of DNAJB6 expression in transfected 293T cell line by DNAJB6 monoclonal antibody (M01), clone 2C11-C1.Lane 1: DNAJB6 transfected lysate(27 KDa).Lane 2: Non-transfected lysate.
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Applications: ELISA, ICC/IF (-), IHC, WB
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Flow Cytometry: HspA4 Antibody (SD0861) [NBP2-67817] - Analysis of Hela cells with HSPA4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: HspA4 Antibody (SD0861) [NBP2-67817] - Staining HSPA4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: HspA4 Antibody (SD0861) [NBP2-67817] - Staining HSPA4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Applications: Flow, ICC/IF, IHC, IHC-P, WB
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NBP1-78785
Western Blot: MDG1 Antibody [NBP1-78785] - Staining of Human Liver (A), Kidney (B) and -Mouse Liver (C) lysate (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence. Approx 26-27 kDa band observed in Human Liver and Kidney lysates and approx. 28 kDa in Mouse Liver lysates (calculated MW of 25.5 kDa according to Human NP_036460.1 and 25.6 kDa according to Mouse NP_038788.2).
Immunocytochemistry/Immunofluorescence: MDG1 Antibody [NBP1-78785] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/Immunofluorescence: MDG1 Antibody [NBP1-78785] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - GR94 in human primary fibroblasts, ER staining. Primary antibody incubation: 1:250 in 10% Serum in PBST (0.1% Triton) O/N 4C. Secondary: Donkey anti-rat 647 1:500 2h RT. ICC/IF image submitted by a verifed customer review.
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Immunohistochemistry-Paraffin: DNAJB4 Antibody [NBP1-81735] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: DNAJB4 Antibody [NBP1-81735] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.
Species: Hu
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Flow Cytometry: GADD153/CHOP Antibody (9C8) [NB600-1335] - An intracellular stain was performed on SK-MEL-28 cells with GADD153/CHOP Antibody (9C8) NB600-1335 (blue) and a matched isotype control MAB004 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).
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NBP2-48704
Western Blot: DNAJC3 Antibody [NBP2-48704] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: DNAJC3 Antibody [NBP2-48704] - Staining of human thyroid gland shows cytoplasmic positivity in glandular cells.
Immunocytochemistry/Immunofluorescence: DNAJC3 Antibody [NBP2-48704] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
hnRNP AB Antibody
NBP2-16865
Western Blot: hnRNP AB Antibody [NBP2-16865] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE w diluted at 1:1000
Knockout Validated: hnRNP AB Antibody [NBP2-16865] - Wild-type (WT) and hnRNP AB knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP AB antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: hnRNP AB Antibody [NBP2-16865] - A549 xenograft, using HNRNPAB antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Species: Hu
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, KO, WB
BOB1 Antibody
(BOB1/2424)
NBP2-75752
Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Mouse Monoclonal Antibody (BOB1/2424).
Western Blot: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Analysis of Raji cell lysate using BOB1 Mouse Monoclonal Antibody (BOB1/2424).
Flow Cytometry: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. BOB1 Antibody (BOB1/2424).; followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PA, WB
DNAJC1 Antibody
NBP1-32448
Immunocytochemistry/Immunofluorescence: DNAJC1 Antibody [NBP1-32448] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: DNAJC1 Antibody [NBP1-32448] - Sample(30 ug whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.
Species: Hu
Applications: ICC/IF, WB
Prosurfactant
Protein C Antibody
NBP1-87201
Western Blot: Prosurfactant Protein C Antibody [NBP1-87201] - Analysis in human lung tissue.
Immunohistochemistry-Paraffin: Prosurfactant Protein C Antibody [NBP1-87201] - Staining of human kidney shows no positivity in cells in glomeruli as expected.
Immunohistochemistry-Paraffin: Prosurfactant Protein C Antibody [NBP1-87201] - Staining of human lung shows moderate to strong cytoplasmic positivity in respiratory epithelial cells.
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Applications: IHC, IHC-P, WB
ATF6 Antibody
(70B1413.1) - BSA Fre
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Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Hypoxia leads to EMT and ER-stress in CRC cells. B. C. Confluent growing SW480 (B) and HCT116 (C) cells were cultured under conditions of normoxia or hypoxia-like conditions (serum free; 100 uM CoCl2, 1-9 h. B-actin (B-act) as loading control. HIF1a was detectable after 3 h of CoCl2 incubation, the amount of the 50 kD-ATF6 fragment, was already enhanced after 1 h of addition of CoCl2. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0087386) licensed under a CC-BY licence.
Flow (Intracellular): ATF6 Antibody (70B1413.1) [NBP1-40256] - An intracellular stain was performed on HeLa cells with ATF6 Antibody (70B1413.1) NBP1-40256F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.. Using the FITC format of this antibody.
Immunohistochemistry-Paraffin: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of ATF6 antibody on Human placenta. Fixed paraffin-embedded sections. Antibody dilution 1:50. Incubated overnight in 4C. Image from verified customer review.
(3 Reviews)
(218 Publications)
Species: Hu, Mu, Pl, Po, Rb, Rt
Applications: ChIP, CyTOF-ready, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, WB
XBP1 Antibody -
BSA Free
NBP1-77681
Immunohistochemistry: XBP1 Antibody [NBP1-77681] - Staining of XBP1 in mouse bladder using DAB with hematoxylin counterstain.
Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on HepG2 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on NIH3T3 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
(8 Publications)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
SEC61A Antibody
NBP1-31267
Immunocytochemistry/Immunofluorescence: SEC61A Antibody [NBP1-31267] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SEC61A Antibody [NBP1-31267] - Cal27 xenograft, using SEC61A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: SEC61A Antibody [NBP1-31267] - Whole cell extracts and extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC61A1 antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
hnRNP-Q Antibody
(1R0G5)
NBP3-16838
Immunohistochemistry-Paraffin: hnRNP-Q Antibody (1R0G5) [NBP3-16838] - Immunohistochemistry of paraffin-embedded human liver cancer using hnRNP-Q /SYNCRIP Rabbit mAb (NBP3-16838) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: hnRNP-Q Antibody (1R0G5) [NBP3-16838] - Immunohistochemistry of paraffin-embedded mouse brain using hnRNP-Q /SYNCRIP Rabbit mAb (NBP3-16838) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/Immunofluorescence: hnRNP-Q Antibody (1R0G5) [NBP3-16838] - Immunofluorescence analysis of C6 cells using hnRNP-Q /SYNCRIP Rabbit mAb (NBP3-16838) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
ACF Antibody
NBP1-90271
Western Blot: ACF Antibody [NBP1-90271] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: ACF Antibody [NBP1-90271] - Staining of human colon, kidney, liver and tonsil using Anti-A1CF antibody NBP1-90271 (A) shows similar protein distribution across tissues to independent antibody NBP2-33412 (B).
Immunohistochemistry-Paraffin: ACF Antibody [NBP1-90271] - Staining of human tonsil shows no positivity as expected.
Species: Hu
Applications: IHC, IHC-P, WB

Publications for DNAJB11 Antibody (NBP1-84899)(1)


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Bioinformatics

Gene Symbol DNAJB11