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DNAJB11 Antibody

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Western Blot: DNAJB11 Antibody [NBP1-84899] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Western Blot: DNAJB11 Antibody [NBP1-84899] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: DNAJB11 Antibody [NBP1-84899] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: DNAJB11 Antibody [NBP1-84899] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: DNAJB11 Antibody [NBP1-84899] - Analysis in human cell line HeLa.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DNAJB11 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKVYNGLQ
Specificity
Specificity of human, mouse, rat DNAJB11 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNAJB11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DNAJB11 Protein (NBP1-84899PEP)
Publications
Read Publication using NBP1-84899.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DNAJB11 Antibody

  • ABBP-2
  • APOBEC1-binding protein 2
  • DnaJ (Hsp40) homolog, subfamily B, member 11
  • dnaJ homolog subfamily B member 11
  • DnaJ protein homolog 9
  • EDJABBP2
  • ER-associated dnaJ protein 3
  • ER-associated DNAJ
  • ER-associated Hsp40 co-chaperone
  • ERdj3DJ9
  • ERJ3
  • ERj3p
  • hDj9
  • hDj-9
  • HEDJERj3
  • Human DnaJ protein 9
  • PRO1080
  • PWP1-interacting protein 4
  • UNQ537

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-GPR78 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - NIH-3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-GRP78/HSPA5 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on HeLa with GRP78/HSPA5 Antibody NBP1-06274 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
(1 Review)
(15 Publications)
Species: Hu, Mu, Rt, Ch, Dr, Sh
Applications: WB, Simple Western, Flow, ICC/IF
HSP40/DNAJB1
Antibody
NBP2-38988
Western Blot: HSP40/DNAJB1 Antibody [NBP2-38988] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunocytochemistry/Immunofluorescence: HSP40/DNAJB1 Antibody [NBP2-38988] - Immunofluorescent staining of human cell line A549 shows localization to nucleus.
Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody [NBP2-38988] - Staining in human adrenal gland and duodenum tissues using anti-DNAJB1 antibody. Corresponding DNAJB1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
BMP-3b/GDF-10
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AF1543
BMP-3b/GDF-10 Antibody [Unconjugated]
(1 Publication)
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Applications: WB, IHC
DNAJA2 Antibody
(OTI3A10)
NBP2-02996
Western Blot: DNAJA2 Antibody (3A10) [NBP2-02996] Analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJA2 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJA2.
Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Human thyroid tissue using anti-DNAJA2 mouse monoclonal antibody.
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
DNAJB6 Antibody
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H00010049-M01
Western Blot: DNAJB6 Antibody (2C11-C1) [H00010049-M01] - DNAJB6 monoclonal antibody (M01), clone 2C11-C1 Analysis of DNAJB6 expression in Jurkat.
Immunocytochemistry/Immunofluorescence: DNAJB6 Antibody (2C11-C1) [H00010049-M01] - Analysis of monoclonal antibody to DNAJB6 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: DNAJB6 Antibody (2C11-C1) [H00010049-M01] - DNAJB6 monoclonal antibody (M01), clone 2C11-C1. Analysis of DNAJB6 expression in C32.
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Applications: WB, ELISA, ICC/IF
HspA4 Antibody
NBP1-81696
Western Blot: HspA4 Antibody [NBP1-81696] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: HspA4 Antibody [NBP1-81696] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HspA4 Antibody [NBP1-81696] - Staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
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MDG1 Antibody
NBP1-87732
Immunohistochemistry-Paraffin: MDG1 Antibody [NBP1-87732] - Staining of human placenta shows positivity in trophoblastic cells.
Immunohistochemistry: MDG1 Antibody [NBP1-87732] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: MDG1 Antibody [NBP1-87732] - Staining of human cerebral cortex shows positivity in neurons.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
gp96/HSP90B1/GRP94
Antibody (9G10)
NB300-619
Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates.
Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in NIH-3T3 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse breast tissue.
Species: Hu, Mu, Rt, Bv, Ch, Ha
Applications: WB, ICC/IF, IHC, IHC-P, IP
DNAJB4 Antibody
NBP1-81735
Western Blot: DNAJB4 Antibody [NBP1-81735] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: DNAJB4 Antibody [NBP1-81735] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: DNAJB4 Antibody [NBP1-81735] - Staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
GADD153/CHOP
Antibody (9C8)
NB600-1335
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - IHC analysis of a formalin fixed and paraffin embedded (FFPE) tissue section of mouse brain using 1:100 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Western blot analysis of GADD153/CHOP expression in HeLa cells treated with 2.5ug/ml tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).
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(20 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP
DNAJC3 Antibody
NBP2-48704
Western Blot: DNAJC3 Antibody [NBP2-48704] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Immunocytochemistry/Immunofluorescence: DNAJC3 Antibody [NBP2-48704] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: DNAJC3 Antibody [NBP2-48704] - Staining of human thyroid gland shows cytoplasmic positivity in glandular cells.
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Western Blot: hnRNP AB Antibody [NBP1-91999] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: hnRNP AB Antibody [NBP1-91999] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: hnRNP AB Antibody [NBP1-91999] - Staining of human uterus, post-menopause shows strong nuclear positivity in glandular cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
BOB1 Antibody
(OTI2D4)
NBP2-45374
Western Blot: BOB1 Antibody (2D4) [NBP2-45374] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BOB1.
Immunohistochemistry: BOB1 Antibody (2D4) [NBP2-45374] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BOB1 Antibody (2D4) [NBP2-45374] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications: WB, IHC
DNAJC1 Antibody
NBP1-85002
Western Blot: DNAJC1 Antibody [NBP1-85002] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-166
Immunohistochemistry-Paraffin: DNAJC1 Antibody [NBP1-85002] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.
Species: Hu
Applications: WB, IHC, IHC-P
Prosurfactant
Protein C Antibody
NBP1-87201
Western Blot: Prosurfactant Protein C Antibody [NBP1-87201] - Analysis in human lung tissue.
Immunohistochemistry-Paraffin: Prosurfactant Protein C Antibody [NBP1-87201] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Prosurfactant Protein C Antibody [NBP1-87201] - Staining of human lung shows high expression.
Species: Hu
Applications: WB, IHC, IHC-P
ATF6 Antibody
(70B1413.1)
NBP1-40256
Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of ATF6. Lane 1, 293 cells transfected with full-length ATF6.* Lane 2, 293 cells transfected with partial length ATF6 (amino acids 1-373).* Lane 3, Untransfected 293 cells. Western blots were probed with 4 ug/ml of the ATF6 monoclonal antibody and visualized with PicoTect Western Blot Chemiluminescence Substrate (10087K). Film was exposed for 1 min. The top arrow corresponds to the ~90 kDa form of ATF6 described as full-length in the literature. *The human full-length and partial length ATF6 plasmids are described in Luo and Lee (2002).
Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Image of anti-ATF6. Whole cell protein from Hek293 and HepG2 treated with and without 10 mM DTT was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-ATF6 and detected with an anti-mouse HRP secondary antibody using a maximum sensitivity ECL reagent. Note the loss of the 90 kDa modified ATF6 protein (arrowhead) upon DTT treatment to activate the unfolded protein response.
Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of ATF6 in mouse liver tissue using 3 ug/ml of ATF6 antibody and 0.25 ug/ml of GAPDH antibody. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction. The ATF6 band may represent under glycosylated or cleaved/active ATF6.
(1 Review)
(149 Publications)
Species: Hu, Mu, Rt, Po, Fi, Ha, Rb
Applications: WB, ChIP, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
XBP1 Antibody
NBP1-77681
Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-XBP1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrow delineates XBP1s and arrowhead XBP1u.
Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77681] - XBP1 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: XBP1 Antibody [NBP1-77681] - Staining of XBP1 in mouse bladder using DAB with hematoxylin counterstain.
(1 Publication)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
SEC61A Antibody
NBP1-31267
Western Blot: SEC61A Antibody [NBP1-31267] - Whole cell extracts and extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC61A1 antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: SEC61A Antibody [NBP1-31267] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SEC61A Antibody [NBP1-31267] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
hnRNP-Q Antibody
NBP2-34068
Western Blot: hnRNP-Q Antibody [NBP2-34068] - Analysis in human cell line Daudi.
Immunocytochemistry/Immunofluorescence: hnRNP-Q Antibody [NBP2-34068] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: hnRNP-Q Antibody [NBP2-34068] - Staining in human thyroid gland and pancreas tissues using anti-SYNCRIP antibody. Corresponding SYNCRIP RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
ACF Antibody
NBP1-90271
Western Blot: ACF Antibody [NBP1-90271] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: ACF Antibody [NBP1-90271] - Staining of human cell line A-431 shows positivity in cytoplasm.
Immunohistochemistry-Paraffin: ACF Antibody [NBP1-90271] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for DNAJB11 Antibody (NBP1-84899)(1)


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Bioinformatics

Gene Symbol DNAJB11