DNA Primase small subunit Antibody - BSA Free

Western Blot: DNA Primase small subunit Antibody - BSA Free [NBP3-46896] - K-562 cells were subjected to SDS PAGE followed by western blot with (DNA Primase small subunit antibody) at dilution of 1:500

Immunoprecipitation: DNA Primase small subunit Antibody - BSA Free [NBP3-46896] - IP Result of anti-DNA Primase small subunit (IP:, 4ug; Detection: 1:300) with HeLa cells lysate 2800ug.

Immunohistochemistry: DNA Primase small subunit Antibody - BSA Free [NBP3-46896] - Immunohistochemistry of paraffin-embedded human lymphoma using (DNA Primase small subunit antibody) at dilution of 1:100

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DNA Primase small subunit Antibody - BSA Free Summary

• Immunogen: primase, DNA, polypeptide 1(49kDa). (Uniprot# P49642)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRIM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for DNA Primase small subunit Antibody - BSA Free

• DNA primase 1
• DNA primase 49 kDa subunit
• DNA primase small subunit
• DNA primase subunit 48
• EC 2.7.7
• EC 2.7.7.-
• MGC12308
• p49
• primase p49 subunit
• primase polypeptide 1, 49kDa
• primase, DNA, polypeptide 1 (49kDa)

Background

DNA Primase small subunit, also known by its gene name PRIM1, is a heterodimer that is composed of a small and large subunit. PRIM1 is 420 amino acids long and approximately 49kDa. The DNA Primase small subunit synthesizes RNA primers for the Okazaki fragments created during discontinuous DNA replication. Current research surrounding PRIM1 has shown a possible link with carcinomas, malaria, neuronitis, and osteosarcoma. DNA Primase small subunit has also been shown to interact with Histone cluster 1 H4/A, H4/B, H4/C, H4/D and H4/E in pathways such as DNA replication, DNA strand elongation, polymerase switching, and lagging strand synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRIM1