DNA Polymerase epsilon catalytic subunit A Antibody

Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA Polymerase epsilon catalytic subunit A antibody (NBP3-13067) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunocytochemistry/Immunofluorescence: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] - DNA Polymerase epsilon catalytic subunit A antibody detects DNA Polymerase epsilon catalytic subunit A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA Polymerase epsilon catalytic subunit A protein stained by DNA Polymerase epsilon catalytic subunit A antibody (NBP3-13067) diluted at 1:2500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody [NBP3-13067] - DNA Polymerase epsilon catalytic subunit A antibody detects DNA Polymerase epsilon catalytic subunit A protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. DNA Polymerase epsilon catalytic subunit A antibody (NBP3-13067) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DNA Polymerase epsilon catalytic subunit A Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human DNA Polymerase epsilon catalytic subunit A. The exact sequence is proprietary.
• Predicted Species: Rat (90%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLE
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:2500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 262 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for DNA Polymerase epsilon catalytic subunit A Antibody

• DKFZp434F222
• DNA polymerase epsilon catalytic subunit A
• DNA polymerase II subunit A
• EC 2.7.7
• EC 2.7.7.7
• POLE1FLJ21434
• polymerase (DNA directed), epsilon

Background

Participates in DNA repair and in chromosomal DNA replication

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLE