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DLGAP2 Antibody

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Western Blot: DLGAP2 Antibody [NBP2-42835] - Analysis of 50 ug whole cell lysate. A: mouse brain 7.5% SDS PAGE diluted at 1:1000.
Western Blot: DLGAP2 Antibody [NBP2-42835] - Analysis of 50 ug whole cell lysate. A: mouse brain 7.5% SDS PAGE diluted at 1:1000.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DLGAP2 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse Dlgap2. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLGAP2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
119 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for DLGAP2 Antibody

  • discs, large (Drosophila) homolog-associated protein 2
  • SAP90/PSD-95-associated protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-41404
Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.
Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).
(1 Review)
(6 Publications)
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
Growth Hormone R
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Growth Hormone R/GHR was detected in perfusion fixed frozen sections of mouse liver using Mouse Growth Hormone R/GHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1360) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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IGF-I/IGF-1
[Unconjugated]
291-G1
1 μg/lane of Recombinant Human IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5 ng/mL.
MALDI-TOF analysis of Recombinant Human IGF-I/IGF-1.The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.
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GFAP Antibody
NB300-141
Immunohistochemistry: GFAP Antibody [NB300-141] - Analysis of a rat cerebellum section stained with rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green and mouse monoclonal antibody to MeCP2, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 uM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.
Western Blot: GFAP Antibody [NB300-141] - Analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50 kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.
Western Blot: GFAP Antibody [NB300-141] - Analysis of Rat brain lysate. Antibody at 1:5000. Specific band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP was observed.
(12 Reviews)
(83 Publications)
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
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PISD Antibody
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Immunohistochemistry: PISD Antibody (4G5) [NBP2-46421] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: PISD Antibody (4G5) [NBP2-46421] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Western Blot: PISD Antibody (4G5) [NBP2-46421] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PISD .
Species: Hu, Mu, Rt
Applications: IHC, WB
PSD-95 Antibody
(6G6-1C9) - BSA Free
NB300-556
Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Mixed 3 week old rat cortical cultures (21 DIV) were labelled with anti-PSD95 (1:650) and visualised with anti-mouse Alexa647 (1:2000). The antibody labels small punctae along neuronal processes as expected. This image was submitted via customer Review.
Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Control immunolocalization of plasma membrane AMPA receptor subunits (external epitopes) in hippocampal neuronal cultures. H) Immunolabeling of non-permeabilized neuronal cultures with ani-GluA1 (green). (I) Immunolabeling with the postsynaptic marker anti-PSD95 [66] after permeabilization of neuronal cultures. (J) Merging of (H) and (I) showing postsynaptic labeling of GluA1. Scale bar (valid for all images): 10 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0140868), licensed under a CC-BY licence.
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Species: Hu, In, Mu, Pm, Rt
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NB100-1028
Immunohistochemistry: AIF-1/Iba1 Antibody [NB100-1028] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Immunohistochemistry: AIF-1/Iba1 Antibody [NB100-1028] - (6ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Western Blot: AIF-1/Iba1 Antibody [NB100-1028] - (0.1ug/ml) staining of Mouse Brain (A), (1ug/ml) Mouse Lymph node (B) and (0.3ug/ml) Rat Brain (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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(267 Publications)
Species: Ca, Fe, Hu, Mu, Rt
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PSD Antibody
H00005662-B01P
Western Blot: PSD Antibody [H00005662-B01P] - Western Blot analysis of PSD expression in transfected 293T cell line (H00005662-T01) by PSD MaxPab polyclonal antibody. Lane1:PSD transfected lysate(112.64 KDa). Lane2:Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: PSD Antibody [H00005662-B01P] - Analysis of purified antibody to PSD on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: PSD Antibody [H00005662-B01P] - Analysis of PSD expression in transfected 293T cell line by PSD polyclonal antibody. Lane1:PSD transfected lysate(112.64 KDa). Lane2:Non-transfected lysate.
Species: Hu, Rt
Applications: ICC/IF, WB
GKAP/DLGAP1
Antibody
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Western Blot: GKAP/DLGAP1 Antibody [NBP3-13181] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GKAP/DLGAP1 antibody (NBP3-13181) diluted at 1:3000.
Western Blot: GKAP/DLGAP1 Antibody [NBP3-13181] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GKAP/DLGAP1 antibody (NBP3-13181) diluted at 1:3000.
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NB120-3344
Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NB120-3344] - Rat hippocampal neurons
Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NB120-3344]
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Species: Hu, Mu, Rt
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Flow Cytometry: FMRP Antibody (1C6) [NBP2-01770] - Analysis of Jurkat cells, using anti-FMRP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: FMRP Antibody (1C6) [NBP2-01770] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FMRP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FMRP.
Immunohistochemistry-Paraffin: FMRP Antibody (1C6) [NBP2-01770] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FMRP mouse monoclonal antibody.
(1 Review)
(2 Publications)
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MCPH1 Antibody
AF3998
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human MCPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MCPH1 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
(1 Review)
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PSD-95 Antibody
(6G6-1C9) - BSA Free
NB300-556
Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Mixed 3 week old rat cortical cultures (21 DIV) were labelled with anti-PSD95 (1:650) and visualised with anti-mouse Alexa647 (1:2000). The antibody labels small punctae along neuronal processes as expected. This image was submitted via customer Review.
Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Control immunolocalization of plasma membrane AMPA receptor subunits (external epitopes) in hippocampal neuronal cultures. H) Immunolabeling of non-permeabilized neuronal cultures with ani-GluA1 (green). (I) Immunolabeling with the postsynaptic marker anti-PSD95 [66] after permeabilization of neuronal cultures. (J) Merging of (H) and (I) showing postsynaptic labeling of GluA1. Scale bar (valid for all images): 10 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0140868), licensed under a CC-BY licence.
(1 Review)
(26 Publications)
Species: Hu, In, Mu, Pm, Rt
Applications: B/N, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
CNPase Antibody
(CL2887)
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Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617] - Staining of rat cerebral cortex shows strong immunoreactivity in single oligodendrocytes.
Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617] - Staining of mouse cerebral cortex shows strong immunoreactivity in oligodendrocytes.
Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617] - Staining of human cerebral cortex shows strong immunoreactivity in oligodendrocytes.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Hemoglobin beta
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NBP2-14081
Immunocytochemistry/Immunofluorescence: Hemoglobin beta Antibody [NBP2-14081] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Hemoglobin beta Antibody [NBP2-14081] - Staining of human placenta shows erythrocyte positivity in vessels.
(1 Review)
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APC Antibody - BSA
Free
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Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: APC Antibody [NB100-91662] - An intracellular stain was performed on HeLa cells with APC Antibody NB100-91662AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - Paraffin-embedded human colon carcinoma tissue.
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TREM2 Antibody
[Unconjugated]
AF1729
TREM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
(1 Review)
(24 Publications)
Species: Mu
Applications: ELISA, ICC, KO, WB
DLGAP2 Antibody
NBP2-42835
DLGAP2 Antibody
Species: Mu
Applications: WB
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol DLGAP2